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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제8호
- 발행연도
- 2006.1
- 수록면
- 1,311 - 1,315 (5page)
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초록· 키워드
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Aminocyclitol antibiotic validamycin A, a primecontrol agent for sheath blight disease of rice plants, isbiosynthesized by Streptomyces hygroscopicus var. limoneus.Within the validamycin biosynthetic gene cluster, vldBC formsan operon of vldABC with vldA, the gene encoding 2-epi-5-epi-valiolone synthase. Biochemical studies, employing therecombinant proteins from Escherichia coli, established VldBand VldC as D-glucose α-1-phosphate uridylyltransferase andglucokinase, respectively. This finding substantiates that thevalidamycin biosynthetic gene cluster harbors genes encoding theenzymes for UDP-glucose formation from glucose. Therefore,we propose that validamycin biosynthesis employs its owncatalysts to generate UDP-glucose, but not depending on theprimary metabolism.
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참고문헌 (22)
참고문헌 신청
Andreone, T. L / 1989 / The amino acid sequence of rat liver glucokinase deduced from cloned cDNA / J. Biol. Chem. 264 : 363 ~ 369
Asano, N / 1987 / Effect of validamycins on glycohydrolases of Rhizoctonia solani / J. Antibiot. 40 : 526 ~ 532
Beyer, S / 1998 / The StrQ protein encoded in the gene cluster for 5'-hydroxystreptomycin of Streptomyces glaucescens GLA.0 is a α-D-glucose-1- phosphate cytidylyltransferas
Brown, K / 1999 / Crystal structure of the bifunctional N-acetylglucosamine 1-phosphate uridyltransferase from Escherichia coli: A paradigm for the related pyrophosphorylase
Dong, H / 2001 / Biosynthesis of the validamycins: Identification of intermediates in the biosynthesis of validamycin A by Streptomyces hygroscopicus var. limoneus / J. Am. C
Asano, N / 1987 / Effect of validamycins on glycohydrolases of Rhizoctonia solani / J. Antibiot. 40 : 526 ~ 532
Beyer, S / 1998 / The StrQ protein encoded in the gene cluster for 5'-hydroxystreptomycin of Streptomyces glaucescens GLA.0 is a α-D-glucose-1- phosphate cytidylyltransferas
Brown, K / 1999 / Crystal structure of the bifunctional N-acetylglucosamine 1-phosphate uridyltransferase from Escherichia coli: A paradigm for the related pyrophosphorylase
Dong, H / 2001 / Biosynthesis of the validamycins: Identification of intermediates in the biosynthesis of validamycin A by Streptomyces hygroscopicus var. limoneus / J. Am. C
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