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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제4호
- 발행연도
- 2012.1
- 수록면
- 470 - 478 (9page)
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초록· 키워드
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Recent genome comparisons of E. coli B and K-12 strains have indicated that the makeup of the cell envelopes in these two strains is quite different. Therefore, we analyzed and compared the envelope proteomes of E. coli BL21(DE3) and MG1655. A total of 165 protein spots,including 62 nonredundant proteins, were unambiguously identified by two-dimensional gel electrophoresis and mass spectrometry. Of these, 43 proteins were conserved between the two strains, whereas 4 and 16 strain-specific proteins were identified only in E. coli BL21(DE3) and MG1655, respectively. Additionally, 24 proteins showed more than 2-fold differences in intensities between the B and K-12 strains. The reference envelope proteome maps showed that E. coli envelope mainly contained channel proteins and lipoproteins. Interesting proteomic observations between the two strains were as follows: (i) B produced more OmpF porin with a larger pore size than K-12,indicating an increase in the membrane permeability; (ii)B produced higher amounts of lipoproteins, which facilitates the assembly of outer membrane β-barrel proteins; and (iii)motility- (FliC) and chemotaxis-related proteins (CheA and CheW) were detected only in K-12, which showed that E. coli B is restricted with regard to migration under unfavorable conditions. These differences may influence the permeability and integrity of the cell envelope, showing that E. coli B may be more susceptible than K-12 to certain stress conditions. Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies.
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참고문헌 (22)
참고문헌 신청
Archer, C. T. / 2011 / The genome sequence of E. coli W (ATCC 9637): Comparative genome analysis and an improved genome-scale reconstruction of E. coli / BMC Genomics 12 : 9
Bagos, P. G. / 2004 / PRED-TMBB: A Web server for predicting the topology of β-barrel outer membrane proteins / Nucleic Acids Res 32 : W400 ~ W404
Borgstrom, B. / 1974 / Bile salts - their physiological functions in the gastrointestinal tract / Acta Med. Scand 196 : 1 ~ 10
Bos, M. P. / 2007 / Biogenesis of the Gram-negative bacterial outer membrane / Annu. Rev. Microbiol 61 : 191 ~ 214
Brown, J. L. / 1997 / Acid habituation of Escherichia coli and the potential roleof cyclopropane fatty acids in low pH tolerance / Int. J. FoodMicrobiol 37 : 163 ~ 173
Bagos, P. G. / 2004 / PRED-TMBB: A Web server for predicting the topology of β-barrel outer membrane proteins / Nucleic Acids Res 32 : W400 ~ W404
Borgstrom, B. / 1974 / Bile salts - their physiological functions in the gastrointestinal tract / Acta Med. Scand 196 : 1 ~ 10
Bos, M. P. / 2007 / Biogenesis of the Gram-negative bacterial outer membrane / Annu. Rev. Microbiol 61 : 191 ~ 214
Brown, J. L. / 1997 / Acid habituation of Escherichia coli and the potential roleof cyclopropane fatty acids in low pH tolerance / Int. J. FoodMicrobiol 37 : 163 ~ 173
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