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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 573 - 578 (6page)
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초록· 키워드
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Pyrimidine nucleotide Nnucleotide phosphoribo(deoxyribo)hydrolase/pyrimidine 5'-nucleotide nucleosidase, EC 3.2.10) catalyzes the breakdownof pyrimidine 5'-nucleotide into pyrimidine base andribose(deoxyribo)-5-phosphate. However, detailed characteristicsof the enzyme have not yet been reported. The enzyme waspurified to homogeneity 327.9-fold with an overall yieldof 6.1% from Pseudomonas oleovorans ATC 8062. Themonophosphate (UMP), but not adenosine monophosphate(AMP) and guanosine monophosphate (GMP). The enzymeoptimaly metabolized CMP at pH 6.0 and UMP at around8.5, and the optimum temperature for the overall enzymereaction was found to be 37oC. The Km values of the enzymefor CMP (at pH 6.0) and UMP (at pH 8.5) were 1.6 mM andvery effective inhibitors of the reaction. Double-reciprocalplots obtained in the absence and in the presence of AMPrevealed that this inhibitory effect was of the mixed competitivetype with respect to the breakdown of CMP and of thenoncompetitive type with respect to the breakdown of UMP.In the presence of AMP, the enzyme followed sigmoidkinetics with respect to each substrate.
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참고문헌 (15)
참고문헌 신청
Agarwal, D. P / 1963 / Improved paper-chromatographic separation of sugar phosphates by using borate-impregnated paper / Analyst 88 : 969 ~ 970
Davis, B. J / 1964 / Disc electrophoresis. 2. Method and application to human serum proteins / Ann. N. Y. Acad. Sci. 121 : 404 ~ 427
Imada, A / 1967 / Degradation of pyrimidine nucleotides by enzyme systems of Streptomyces. I. Ribose-5-phosphate formation from pyrimidine nucleotides / J. Gen. Appl. Microbi
Imada, A / 1967 / Degradation of pyrimidine nucleotides by enzyme systems of Streptomyces. II. Pyrimidine 5'-nucleotide phosphoribo(deoxyribo) hydrolase of Streptomyces virgi
KIM, JUNG / 2004 / Purification and Properties of Intracellular Cytosine Deaminase fromChromobacterium violaceum YK 391 / Journal of Microbiology and Biotechnology 14 (6) : 1
Davis, B. J / 1964 / Disc electrophoresis. 2. Method and application to human serum proteins / Ann. N. Y. Acad. Sci. 121 : 404 ~ 427
Imada, A / 1967 / Degradation of pyrimidine nucleotides by enzyme systems of Streptomyces. I. Ribose-5-phosphate formation from pyrimidine nucleotides / J. Gen. Appl. Microbi
Imada, A / 1967 / Degradation of pyrimidine nucleotides by enzyme systems of Streptomyces. II. Pyrimidine 5'-nucleotide phosphoribo(deoxyribo) hydrolase of Streptomyces virgi
KIM, JUNG / 2004 / Purification and Properties of Intracellular Cytosine Deaminase fromChromobacterium violaceum YK 391 / Journal of Microbiology and Biotechnology 14 (6) : 1
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