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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 477 - 483 (7page)
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초록· 키워드
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Corynebacterium ammoniagenes ATCC 6872, whichdoes not accumulate pyrimidine nucleoside or nucleotide,was metabolically engineered to secrete a large amount ofthymidine. Characteristics of 5-fluorouracil resistance (FUr),hydroxyurea resistance (HUr), trimethoprim resistance (TMr),thymidylate phosphorylase deficiency (deoA-), inosine auxotrophy(ino-), 5-fluorocytosine resistance (FCr), thymidine kinasedeficiency, and thymidine resistance (thymr) were successivelyintroduced into mutant strains KR3 and DY5T9-5, andshake-flask cultures were able to accumulate 408.1 mg/land 428.2 mg/l of thymidine, respectively, as a majorproduct. The mutant strains did not accumulate thymine atall and accumulated less than 10 mg/l of other pyrimidinenucleosides, such as cytosine, cytidine, and deoxycytidine, asbyproducts.
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참고문헌 (12)
참고문헌 신청
Appl / 1994 / Chemostat selection of Escherichia colimutants secreting thymidine : 232 ~ 238
Hammer / 1983 / Metabolism of Nucleotides Nucleosides and Nucleobases in Microorganism : 203 ~
Lowry / 1951 / Protein measurement with the Folin phenolreagent : 265 ~ 275
Neuhard / 1983 / Utilization of preformed pyrimidine basesand nucleoside Metabolism of Nucleotides : 95 ~ 148
Potvin / 1975 / Pyrimidine biosynthetic pathway of Bacillus subtilis : 604 ~ 615
Hammer / 1983 / Metabolism of Nucleotides Nucleosides and Nucleobases in Microorganism : 203 ~
Lowry / 1951 / Protein measurement with the Folin phenolreagent : 265 ~ 275
Neuhard / 1983 / Utilization of preformed pyrimidine basesand nucleoside Metabolism of Nucleotides : 95 ~ 148
Potvin / 1975 / Pyrimidine biosynthetic pathway of Bacillus subtilis : 604 ~ 615
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