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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제4호
- 발행연도
- 2010.1
- 수록면
- 693 - 699 (7page)
이용수
초록· 키워드
오류제보하기
An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa, as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and 60oC, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C>poly G>poly A>poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an IC50 value of 8.6 μM and 7.2 μM, respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.
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참고문헌 (21)
참고문헌 신청
Adinolfi, B. S / 1995 / Full antitumor action of recombinant seminal ribonuclease depends on the removal of its N-terminal methionine / Biochem. Biophys. Res. Commun 213 : 52
Ardelt, W / 2008 / Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes / Curr. Pharm. Biotechnol 9 : 215 ~ 225
Brondz, I / 2004 / Multivariate analysis of fatty acids in spores of higher basidiomycetes: A new method for chemotaxonomical classification of fungi / J. Chromatogr. B Analy
Clericuzio, M / 1998 / The sesquiterpenoid contents of fruit bodies of Russula delica / Acta Chem. Scand 52 : 1333 ~ 1337
Elmastas, M / 2007 / Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms / J. Food Compos. Anal 20 : 337 ~ 345
Ardelt, W / 2008 / Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes / Curr. Pharm. Biotechnol 9 : 215 ~ 225
Brondz, I / 2004 / Multivariate analysis of fatty acids in spores of higher basidiomycetes: A new method for chemotaxonomical classification of fungi / J. Chromatogr. B Analy
Clericuzio, M / 1998 / The sesquiterpenoid contents of fruit bodies of Russula delica / Acta Chem. Scand 52 : 1333 ~ 1337
Elmastas, M / 2007 / Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms / J. Food Compos. Anal 20 : 337 ~ 345
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