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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제4호
- 발행연도
- 2010.1
- 수록면
- 700 - 707 (8page)
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초록· 키워드
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A novel antioxidative peptide (APVPH I, antioxidative peptides from venison protein hydrolysates I) was purified from venison by enzymatic hydrolysis, column chromatography of DEAE-Sephacel, and high-performance liquid chromatography. The molecular mass of the purified peptide was found to be 9,853 Da and the amino acid sequences of the purified peptide was Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly. The purpose of this study was to evaluate the effects of APVPH I against H2O2-induced neuronal cells damage in PC-12 cells. Antioxidative enzyme levels in cultured neuronal cells were increased in the presence of the peptide. In addition, APVPH I inhibited productions of nitric oxide (NO), reactive oxygen species (ROS), malondialdehyde (MDA), and cell death against H2O2-induced neuronal cell damage in PC-12 cells. It was presumed to be APVPH I involved in regulating the apoptosis-related gene expression in the cell environment.
The present results indicate that APVPH I substantially contributes to antioxidative properties in neuronal cells.
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참고문헌 (25)
참고문헌 신청
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Bernardi, P / 1994 / Recent progress on regulation of the mitochondrial permeability transition pore: A cyclosporine-sensitive pore in the inner mitochondrial membrane / J. B
Brandt, R / 1965 / Synthesis of diacetylchlorofluorescin: A stable reagent for fluorometric analysis / Anal. Biochem 11 : 6 ~ 9
Brown, G. C / 2003 / Inflammatory neurodegeneration mediated by nitric oxide, glutamate, and mitochondria / Mol. Neurobiol 27 : 325 ~ 355
Bass, D. A / 1983 / Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation / J. Immunol 130 : 1910 ~ 1917
Bernardi, P / 1994 / Recent progress on regulation of the mitochondrial permeability transition pore: A cyclosporine-sensitive pore in the inner mitochondrial membrane / J. B
Brandt, R / 1965 / Synthesis of diacetylchlorofluorescin: A stable reagent for fluorometric analysis / Anal. Biochem 11 : 6 ~ 9
Brown, G. C / 2003 / Inflammatory neurodegeneration mediated by nitric oxide, glutamate, and mitochondria / Mol. Neurobiol 27 : 325 ~ 355
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