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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제3호
- 발행연도
- 2009.1
- 수록면
- 331 - 337 (7page)
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초록· 키워드
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Interleukin-13 (IL-13) has been proposed as a therapeutic
target for bronchial asthma as it plays crucial roles in the
pathogenesis of the disease. We developed an in vitro test
system measuring transcriptional downregulatory activities
on IL-13 as a primary screening method to select drug
candidates from natural products. The promoter region of
IL-13 (-2,048 to +1) was cloned into the upstream of a
luciferase gene in the plasmid pGL4.14 containing the
hygromycin resistance gene as a selection marker, generating
pGL4.14-IL-13. The EL-4 thymoma and RBL-2H3 mast
cells transiently expressing this plasmid highly produced
the luciferase activities by responding to PI (PMA and
ionomycin) stimulation up to 8-fold and 13-fold compared
with the control, respectively, whereas cyclosporin A, a wellknown
antiasthmatic agent, significantly downregulated
the activities. The BF1 clone of RBL-2H3 cells constitutively
expressing pGL4.14-IL-13 was established by selecting
surviving cells under a constant lethal dose of hygromycin
treatment. The feasibility of this system was evaluated by
measuring the downregulatory activities of 354 natural
products on the IL-13 promoter using the BF1 clone. An
extract from Morus bombycis (named TBRC 156) significantly
inhibited PI-induced luciferase activities and IL-13 mRNA
expression, but not the protein expression. Fisetin (named
TBRC 353) inhibited not only PI-induced luciferase activities
and mRNA expression, but also the IL-13 protein secretion,
whereas myricetin (named TBRC 354) could not suppress
the IL-13 expression at all. Our data indicated that this in
vitro test system is able to discriminate the effects on IL-13
expression, and furthermore, that it might be suitable as a
simple and time-saving primary screening system to select
antiasthmatic agents by measuring transcriptional activities
of the IL-13 promoter.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Arai, Y. / 2000 / Dietary intakes of flavonols, flavones and isoflavones by Japanese women and the inverse correlation between quercetin intake and plasma LDL cholesterol con
Blanchard, C. / 2005 / Inhibition of human interleukin- 13-induced respiratory and oesophageal inflammation by antihuman- interleukin-13 antibody (CAT-354) / Clin. Exp. Aller
Das, J. / 2001 / A critical role for NF-kappa B in GATA3 expression and TH2 differentiation in allergic airway inflammation / Nat. Immunol 2 : 45 ~ 50
Grunig, G. / 1998 / Requirement for IL-13 independently of IL-4 in experimental asthma / Science 282 : 2261 ~ 2263
Higa, S. / 2003 / Fisetin, a flavonol, inhibits TH2-type cytokine production by activated human basophils / J. Allergy Clin. Immunol 111 : 1299 ~ 1306
Blanchard, C. / 2005 / Inhibition of human interleukin- 13-induced respiratory and oesophageal inflammation by antihuman- interleukin-13 antibody (CAT-354) / Clin. Exp. Aller
Das, J. / 2001 / A critical role for NF-kappa B in GATA3 expression and TH2 differentiation in allergic airway inflammation / Nat. Immunol 2 : 45 ~ 50
Grunig, G. / 1998 / Requirement for IL-13 independently of IL-4 in experimental asthma / Science 282 : 2261 ~ 2263
Higa, S. / 2003 / Fisetin, a flavonol, inhibits TH2-type cytokine production by activated human basophils / J. Allergy Clin. Immunol 111 : 1299 ~ 1306
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