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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 869 - 883 (15page)
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초록· 키워드
오류제보하기
Interleukin (IL)-15 is an essential immune-modulator with high potential for use in cancer treatment. Natural IL-15 has a low biological potency because of its short half-life and difficulties in mass-production. IL-15Rα, a member of the IL-15 receptor complex, is famous for its high affinity to IL-15 and its ability to lengthen the half-life of IL-15. We have double-transfected IL-15 and its truncated receptor IL-15Rα into CT26 colon cancer cells to target them for intracellular assembly. The secreted IL-15:IL-15Rα complexes were confirmed in ELISA and Co-IP experiments. IL-15:IL-15Rα secreting clones showed a higher anti-tumor effect than IL-15 secreting clones. Furthermore, we also evaluated the vaccine and therapeutic efficacy of the whole cancer-cell vaccine using mitomycin C (MMC)-treated IL-15:IL-15Rα secreting CT26 clones. Three sets of experiments were evaluated; (1) therapeutics, (2) vaccination, and (3) long-term protection. Wild-type CT26-bearing mice treated with a single dose of MMC-inactivated secreted IL-15:IL-15Rα clones prolonged survival compared to the control group. Survival of MMC-inactivated IL-15:IL-15Rα clone-vaccinated mice (without any further adjuvant) exceeded up to 100%. This protection effect even lasted for at least three months after the immunization. Secreted IL-15:IL-15Rα clones challenging trigger anti-tumor response via CD4+ T, CD8+ T, and natural killer (NK) cell-dependent cytotoxicity. Our result suggested that cell-based vaccine secreting IL-15:IL-15Rα, may offer the new tools for immunotherapy to treat cancer.
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참고문헌 (47)
참고문헌 신청
Alahari, S. V. / 2015 / Are macrophages in tumors good targets for novel therapeutic approaches? Mol / Cells 38:95~104
Bamford, R. N. / 1998 / The 5’ untranslated region, signal peptide, and the coding sequence of the carboxyl terminus of IL-15 participate in its multifaceted translational control / J. Immunol 160:4418~4426
Bergado Baez, G. / 2018 / HER1-based vaccine : simultaneous activation of humoral and cellular immune response / Semin. Oncol 45:75~83
Bern, M. D. / 2019 / Inducible down-regulation of MHC class I results in natural killer cell tolerance / J. Exp. Med 216:99~116
Bordon, Y. / 2015 / T cell memory : new insight on old-timers / Nat. Rev. Immunol 15:331
Bamford, R. N. / 1998 / The 5’ untranslated region, signal peptide, and the coding sequence of the carboxyl terminus of IL-15 participate in its multifaceted translational control / J. Immunol 160:4418~4426
Bergado Baez, G. / 2018 / HER1-based vaccine : simultaneous activation of humoral and cellular immune response / Semin. Oncol 45:75~83
Bern, M. D. / 2019 / Inducible down-regulation of MHC class I results in natural killer cell tolerance / J. Exp. Med 216:99~116
Bordon, Y. / 2015 / T cell memory : new insight on old-timers / Nat. Rev. Immunol 15:331
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