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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제2호
- 발행연도
- 2008.1
- 수록면
- 248 - 254 (7page)
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Candida magnoliae, an osmotolerant and erythritol producing yeast, prefers D-fructose to D-glucose as carbon sources.
For the investigation of the fructophilic characteristics with respect to sugar transportation, a sequential extraction method using various detergents and ultracentrifugation was developed to isolate cellular membrane proteins in C.
magnoliae. Immunoblot analysis with the Pma1 antibody and two-dimensional electrophoresis analysis coupled with MS showed that the fraction II was enriched with membrane proteins. Eighteen proteins out of 36 spots were identified as membrane or membrane-associated proteins involved in sugar uptake, stress response, carbon metabolism, and so on. Among them, three proteins were significantly upregulated under the fructose supplying conditions. The hexose transporter was highly homologous to Ght6p in Schizosaccharomyces pombe, which was known as a predominant transporter for the fructose uptake of S.
pombe because it exhibited higher affinity to D-fructose than D-glucose. The physicochemical properties of the ATP-binding cassette transporter and inorganic transporter explained their direct or indirect associations with the fructophilic behavior of C. magnoliae. The identification and characterization of membrane proteins involved in sugar uptake might contribute to the elucidation of the selective utilization of fructose to glucose by C. magnoliae at a molecular level.
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참고문헌 (22)
참고문헌 신청
Bagnat, M. / 2000 / Lipid rafts function in biosynthetic delivery ofproteins to the cell surface in yeast. Proc. Natl. Acad / Sci 97 : 3254 ~ 3259
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Brugiere, S. / 2004 / The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions / Phytochemistry 65 : 1693 ~ 1707
CHA, KWANG-HYUN / 2006 / Coexpression of Protein Disulfide Isomerase (PDI) Enhances Production of Kringle Fragment of Human Apolipoprotein(a) in Recombinant Saccharomycescere
Choi, J. H. / 2003 / Effects of fermentation conditions on production of erythritol by Candida magnoliae. Kor. J. Food Sci / Technol 35 : 708 ~ 712
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Brugiere, S. / 2004 / The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions / Phytochemistry 65 : 1693 ~ 1707
CHA, KWANG-HYUN / 2006 / Coexpression of Protein Disulfide Isomerase (PDI) Enhances Production of Kringle Fragment of Human Apolipoprotein(a) in Recombinant Saccharomycescere
Choi, J. H. / 2003 / Effects of fermentation conditions on production of erythritol by Candida magnoliae. Kor. J. Food Sci / Technol 35 : 708 ~ 712
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