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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제6호
- 발행연도
- 2006.1
- 수록면
- 901 - 910 (10page)
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초록· 키워드
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polyester intracellularly accumulated as distinct granules innumerous microrganisms as an energy and carbon storagematerial. Recombinant Escherichia coli harboring the heterologousP(3HB) biosynthesis genes acumulates large amounts ofP(3HB) granules, yet the granule-asociated proteins have notbeen identified. Therefore, this study reports on analysis ofthe P(3HB) granule-associated proteome in recombinant E.coli. Five proteins out of 7 spots identified were found to beinvolved in functions of translation, heat-stress responses, andP(3HB) biosynthesis. Two of the major granule-asociatedproteins, IbpA/B, which are already known to bind to recombinantproteins forming inclusion bodies in E. coli, were furtheranalyzed. Immunoblotting and immunoelectron microscopicstudies with IbpA/B antibodies clearly demonstrated the bindingand localization of IbpA/B to P(3HB) granules. IbpA/B seemedto play an important role in recombinant E. coli producingP(3HB) by stabilizing the interface betwen the hydrophobicB were found to act like phasins in recombinant E. coli, asthey are the major proteins bound to the P(3HB) granules,affect the morphology of the granules, and reduce the amountof cytosolic proteins bound to the P(3HB) granules.
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참고문헌 (20)
참고문헌 신청
Allen, S. P / 1992 / Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coli / J. Bacteriol. 174 : 6938 ~ 694
Basha, E / 2004 / The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellu
Bova, M. P / 2000 / Subunit exchange of small heat shock proteins. Analysis of oligomer formation of alphaA-crystallin and Hsp27 by fluorescence resonance energy transfer and
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem. 7
Braunegg, G / 1978 / A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass / Eur. J. Appl. Microbiol. Biotechnol. 6 : 2
Basha, E / 2004 / The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellu
Bova, M. P / 2000 / Subunit exchange of small heat shock proteins. Analysis of oligomer formation of alphaA-crystallin and Hsp27 by fluorescence resonance energy transfer and
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem. 7
Braunegg, G / 1978 / A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass / Eur. J. Appl. Microbiol. Biotechnol. 6 : 2
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