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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제1호
- 발행연도
- 2006.1
- 수록면
- 145 - 148 (4page)
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초록· 키워드
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A new functional lipolytic enzyme (AT4) has recentlybeen found from Agrobacterium tumefaciens C58 Cereonusing a genome-wide approach. The enzyme has some sequencesimilarity to E. coli acetyl hydrolase, Emericella nidulanslipase, Moraxella sp. lipase, Acinetobacter lwoffi esterase,and Streptomyces hygroscopicus acetyl hydrolase. However,the sequence similarities are very low (less than 25%),suggesting that it is a new lipase/esterase enzyme. In the presentstudy, intact cell of the A. tumefaciens strain was shown towas then expressed at a high level in E. coli BL21 (DE3) cellsand the enzyme was purified simply by Ni-NTA columnchromatography. The purified enzyme showed hydrolyticactivity toward p-nitrophenyl caproate, but not toward oliveoil, suggesting that the AT4 enzyme was a typical esteraserather than lipase. AT4 esterase had a maximum hydrolyticactivity at 45oC and pH 8.0, when p-nitrophenyl caproateoC andat pH 5.0-9.0. Calcium ion and EDTA did not affect theactivity and thermal stability of the enzyme. As for substratespecificity, AT4 enzyme could rapidly hydrolyze acetyl andbutyl groups from p-nitrophenyl esters and 1-naphthyl esters.In adition, it also released acetyl residues from acetylatedglucose and xylose substrates. Therefore, this new esteraseenzyme might be used as a biocatalyst in acetylation and
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참고문헌 (20)
참고문헌 신청
Brown, D. W / 1996 / Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans / Proc. Natl. Acad. Sci. USA 20 : 1418 ~ 1422
Degrassi, G / 1998 / Purification and characterization of an acetyl xylan esterase from Bacillus pumilus / Appl. Environ. Microbiol. 64 : 789 ~ 792
Feller, G / 1991 / Nucleotide sequence of the lipase gene lip2 from the antarctic psychrotroph Moraxella TA144 and site-specific mutagenesis of the conserved serine and histi
Jaeger, K. E / 1999 / Bacterial biocatalysts: Molecular biology, three-dimensional structures, and biotechnological applications of lipases / Annu. Rev. Microbiol. 53 : 315 ~
Jaeger, K. E / 2002 / Lipases for biotechnology / Curr. Opin. Biotechnol 13 : 390 ~ 397
Degrassi, G / 1998 / Purification and characterization of an acetyl xylan esterase from Bacillus pumilus / Appl. Environ. Microbiol. 64 : 789 ~ 792
Feller, G / 1991 / Nucleotide sequence of the lipase gene lip2 from the antarctic psychrotroph Moraxella TA144 and site-specific mutagenesis of the conserved serine and histi
Jaeger, K. E / 1999 / Bacterial biocatalysts: Molecular biology, three-dimensional structures, and biotechnological applications of lipases / Annu. Rev. Microbiol. 53 : 315 ~
Jaeger, K. E / 2002 / Lipases for biotechnology / Curr. Opin. Biotechnol 13 : 390 ~ 397
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