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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제1호
- 발행연도
- 2006.1
- 수록면
- 20 - 24 (5page)
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초록· 키워드
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Plasmid DNA (pDNA) is a product of interest formany biopharmaceutical companies and research laboratories,because of increase in the number of gene therapy protocolsthat use nonviral vectors. This work was undertaken to studythe effect of antibiotic and dissolved oxygen concentration(DOC) on the production of a ColE1-type plasmid (pVAX1-LacZ) hosted in Escherichia coli DH5α and cultured in abatch fermentor with 0.75 l of Terrific Broth. A decreasein the DOC from 60% to 5% was shown to increase thespecific pDNA concentration approximately 1.5-fold, due tothe downregulation of growth. Additionally, this increase inthe pDNA concentration led to a 2.2-fold increase in thepurity of cell lysates obtained after cell lysis. However, the useof higher DOC led to 2.8-fold higher volumetric productivity asa consequence of a faster growth rate, reducing the fermentationtime from 24 to 8 h. Interestingly, the specific pDNAconcentration, and pDNA productivity and purity were alwayshigher (10-15%) in the absence of antibiotic. Overall, thedata indicate that nonselective conditions can be used withoutcompromising yield, productivity, and purity of pDNA.
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참고문헌 (13)
참고문헌 신청
Birnboim, H. C / 1979 / A rapid alkaline extraction procedure for screening recombinant plasmid DNA / Nucleic Acids Res. 7 : 1513 ~ 1523
CBER / 1996 / Points to consider on plasmid DNA vaccines for preventive infectious disease indications / US FDA. Rockville, MD, U.S.A.
Chen, W / 1997 / Automated fed-batch fermentation with feed-back controls based on dissolved oxygen (DO) and pH for production of DNA vaccines / J. Ind. Microbiol. Biotechnol
Chen, W. / 1999 / Automated high-yield fermentation of plasmid DNA in Escherichia coli. American Home Products Corporation / US Patent: 5955323
Diogo, M. M / 2003 / Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography / J. Chromatogr
CBER / 1996 / Points to consider on plasmid DNA vaccines for preventive infectious disease indications / US FDA. Rockville, MD, U.S.A.
Chen, W / 1997 / Automated fed-batch fermentation with feed-back controls based on dissolved oxygen (DO) and pH for production of DNA vaccines / J. Ind. Microbiol. Biotechnol
Chen, W. / 1999 / Automated high-yield fermentation of plasmid DNA in Escherichia coli. American Home Products Corporation / US Patent: 5955323
Diogo, M. M / 2003 / Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography / J. Chromatogr
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