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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제1호
- 발행연도
- 2006.1
- 수록면
- 77 - 83 (7page)
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A gene encoding a γ-butyrolactone autoregulatorreceptor was cloned from , andthe biochemical characteristics, including the autoregulatorspecificity, were determined with the purified recombinantprotein. Using primers designed for the conserved aminoacid sequence of Streptomyces γ-butyrolactone autoregulatorreceptors, a 120 bp S. erythraea DNA fragment was obtainedby PCR. Southern and colony hybridization with the 120 bpfragment as a probe allowed to select a genomic clone of S.erythraeasequencing analysis revealed a 615 bp open reading frame(ORF), showing moderate homology (identity, 31-34%;similarity, 45-47%) with the γ-butyrolactone autoregulatorreceptors from Streptomyces sp., and this ORF was namedseaR (Saccharopolyspora erythraea autoregulator receptor).The seaRrecombinant SeaR protein (rSeaR) in Escherichia coli, andthe rSeaR protein was purified to homogeneity by DEAE-Sephacel column chromatography, folowed by DEAE-ion-exchange HPLC. The molecular mas of the purified rSeaRprotein was 52 kDa by HPLC gel-filtration chromatographyand 27 kDa by SDS-polyacrylamide gel electrophoresis,indicating that the rSeaR protein is present as a dimer. Abinding assay with tritium-labeled autoregulators revealedthat rSeaR has clear binding activity with a VB-C-typethe first time that the erythromycin producer S. erythraeapossesses a gene for the γ-butyrolactone autoregulator receptor.
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참고문헌 (23)
참고문헌 신청
[학술
Bibb, M. J / 1984 / The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequ
Christopher, C / 2002 / Saccharopolyspora erythraeacatalyzed bioconversion of 6-deoxyerythronolide B analogs for production of novel erythromycin / J. Biotechnol. 92 : 217 ~
Grafe, U / 1982 / A new inducer of anthracycline biosynthesis from Streptomyces viridochromogenes / J. Antibiot. 35 : 1722 ~ 1723
Grafe, U / 1983 / Interspecific inducers of cytodifferentiation and anthracycline biosynthesis from Streptomyces bikiniensis and S. cyaneofuscatus / Biotechnol. Lett. 5 : 591
Bibb, M. J / 1984 / The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequ
Christopher, C / 2002 / Saccharopolyspora erythraeacatalyzed bioconversion of 6-deoxyerythronolide B analogs for production of novel erythromycin / J. Biotechnol. 92 : 217 ~
Grafe, U / 1982 / A new inducer of anthracycline biosynthesis from Streptomyces viridochromogenes / J. Antibiot. 35 : 1722 ~ 1723
Grafe, U / 1983 / Interspecific inducers of cytodifferentiation and anthracycline biosynthesis from Streptomyces bikiniensis and S. cyaneofuscatus / Biotechnol. Lett. 5 : 591
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