지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제2호
- 발행연도
- 2005.1
- 수록면
- 376 - 383 (8page)
이용수
초록· 키워드
오류제보하기
Alcaligenes sp. JMP228 carrying 2,4-dichlorophenoxyacetic acid (2,4-D) degradative plasmid pJP4was inoculated into natural soil, and transfer of the plasmidpJP4 to indigenous soil bacteria was investigated with andwithout 2,4-D amendment. Plasmid pJP4 transfer was enhancedin the soils treated with 2,4-D, compared to the soils notamended with 2,4-D. Several different transconjugants wereisolated from the soils treated with 2,4-D, while no indigenoustransconjugants were obtained from the unamended soils.Inoculation of the soils with both the donor Alcaligenessp. JMP228/pJP4 and a recipient Burkholderia cepaciaDBO1 produced less diverse transconjugants than the soilsinoculated with the donor alone. Repetitive extragenicpalindromic-polymerase chain reaction (REP-PCR) analysisof the transconjugants exhibited seven distinct genomic DNAfingerprints. Analysis of 16S rDNA sequences indicatedthat the transconjugants were related to members of thegenera Burkholderia and Pandoraea. Denaturing gradient gelelectrophoresis (DGGE) analysis of PCR-amplified 16S rRNAgenes revealed that inoculation of the donor caused clearchanges in the bacterial community structure of the 2,4-Damendedsoils. The new 16S rRNA gene bands in the DGGEprofile corresponded with the 16S rRNA genes of 2,4-Ddegradingtransconjugants isolated from the soil. The resultsindicate that introduction of the 2,4-D degradative plasmid asAlcaligenes sp. JMP228/pJP4 has a substantial impact on thebacterial community structure in the 2,4-D-amended soil.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
A / 1997 / Plasmid transfer between spatially separated donor andrecipient bacteria in earthworm-containing soil microcosms 63 : 679 ~ 686
Appl / 1992 / Use of repetitive sequences and the polymerase chain reaction to fingerprintthe genomes of Rhizobium meliloti isolates and other soilbacteria : 2180 ~ 2187
Bae / 2003 / Application of denaturing gradient gel electrophoresisto estimate the diversity of commensal thermophiles 13 (6) : 1008 ~ 1012
Balkwill / 1990183-211 / Isolation of BiotechnologicalOrganisms from Nature
Cho / / antagonistic against phytopathogenic fungicausing damping-off disease : 599 ~ 606
Appl / 1992 / Use of repetitive sequences and the polymerase chain reaction to fingerprintthe genomes of Rhizobium meliloti isolates and other soilbacteria : 2180 ~ 2187
Bae / 2003 / Application of denaturing gradient gel electrophoresisto estimate the diversity of commensal thermophiles 13 (6) : 1008 ~ 1012
Balkwill / 1990183-211 / Isolation of BiotechnologicalOrganisms from Nature
Cho / / antagonistic against phytopathogenic fungicausing damping-off disease : 599 ~ 606
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