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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제42권 제1호
발행연도
2014.1
수록면
32 - 40 (9page)

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초록· 키워드

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Methane oxidation and the production potentials of ground soil (soil A) and garden soil (soil B, C, & D) in an urban school wereevaluated, and the methanotrophic and methanogen communities in the soil samples were quantified using quantitative realtimePCR. The methanotrophic community in the raw soil A sample possessed a 6.1×103 gene copy number/g dry weight soil,whereas those in the raw soils B~D samples were 1.6-1.9 × 105 gene copy numbers/g dry weight soil. Serum bottles addedwith the soil samples were enriched with methane gas, and then evaluated for their methane oxidation potential. The soil Asample had a longer induction phase for methane oxidation than the other soils. However, soil A showed a similar methane oxidationpotential with soils B~D after the induction phase. The methanotrophic community in the enriched soil A sample wasincreased by up to 2.3 × 107 gene copy numbers/g dry weight soil, which had no significantly difference compared with those insoils B~D (1.2-2.8 × 108 gene copy numbers/g dry weight soil). Methane production showed a similar tendency to methane oxidation. The methanogens community in raw soil A (1.7 × 105 gene copy number/g dry weight soil) was much less than those inraw soils B~D (1.3-3.4 × 107 gene copy numbers/g dry weight soil). However, after methane gas was produced by addingstarch to the soils, soil samples A~D showed 107 gene copy numbers/g dry weight soil in methanogens communities. Theresults indicate that methanotrophic and methanogenic bacteria have coexisted in this urban school’s soils. Moreover, underappropriate conditions for methane oxidation and production, methanotrophic bacteria and methanogens are increased andthey have the potential for methane oxidation and production.

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