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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제9호
- 발행연도
- 2008.1
- 수록면
- 1,606 - 1,612 (7page)
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초록· 키워드
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Interleukin (IL)-32 is a recently identified proinflammatory
cytokine that is one of the IL-18 inducible genes, and plays
an important role in autoimmune and inflammatory
diseases. We produced antibodies against IL-32 and studied
the expression of IL-32 in human stomach cancer. We
detected IL-32 secreted from K-562 cells which were stably
transfected with IL-32 and in the sera of stomach cancer
patients by a sandwich ELISA using a monoclonal antibody
KU32-52 and a polyclonal antibody. In order to optimize a
sandwich immunoassay, recombinant IL-32α was added,
followed by the addition of a biotinylated KU32-52 into
microtiter plate wells precoated with a goat anti-IL-32
antibody. The bound biotinylated KU32-52 was probed
with a streptavidin conjugated to HRP. This sandwich
ELISA was highly specific and had a minimal detection
limit of 80 pg/ml (mean±SD of zero calibrator) and measuring
up to 3,000 pg/ml. This ELISA showed no cross-reaction
with other cytokines such as hIL-1α, hIL-1β, hIL-2, hIL-6,
hIL-8, hIL-10, hIL-18, and hTNF-α. Intra-assay coefficients
of variation were 18.5% to 4.6% (n=10), and inter-assay
coefficients were 23% to 9% (n=10). The average IL-32 level
in the sera of 16 stomach cancer patients (189 pg/ml) was
higher than that of 12 healthy control men (109 pg/ml).
Our results indicate that serum IL-32 level can be detected
by using an established ELISA, and that this immunoassay
and mAb KU32-09 specific for immunohistochemistry
can be used in the detection of expressed and secreted IL-
32 in stomach cancer patients.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Banda, N. K. / 2003 / Mechanisms of inhibition of collagen-induced arthritis by murine IL-18 binding protein. J / Immunol 170 : 2100 ~ 2105
Bazan, J. F. / 1996 / A newly defined interleukin / Nature 379 (591)
CHO, HYUN-JEONG / 2007 / Inhibitory Effects of Cordycepin (3'-Deoxyadenosine), a Component ofCordyceps militaris, on Human Platelet Aggregation Induced by Thapsigargin / Jour
Cagnard, N. / 2005 / Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis f
Chen, Q. / 2006 / The newest interleukins: Recent additions to the ever-growing cytokine family. Vitam / Horm 74 : 207 ~ 228
Bazan, J. F. / 1996 / A newly defined interleukin / Nature 379 (591)
CHO, HYUN-JEONG / 2007 / Inhibitory Effects of Cordycepin (3'-Deoxyadenosine), a Component ofCordyceps militaris, on Human Platelet Aggregation Induced by Thapsigargin / Jour
Cagnard, N. / 2005 / Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis f
Chen, Q. / 2006 / The newest interleukins: Recent additions to the ever-growing cytokine family. Vitam / Horm 74 : 207 ~ 228
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