지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제8호
- 발행연도
- 2008.1
- 수록면
- 1,357 - 1,363 (7page)
이용수
초록· 키워드
오류제보하기
The PentC promoter of the entCEBA operon encoding enzymes for enterobactin biosynthesis in Escherichia coli is tightly regulated by the availability of iron in the culture medium. In iron-rich conditions, the PentC promoter activity is strongly repressed by the global transcription regulator Fur (ferric uptake regulator), which complexes with ferrous ions and binds to the Fur box 19-bp inverted repeat. In this study, we have constructed the expression vector pOS2 containing the PentC promoter and characterized its repression, induction, and modulation by quantifying the expression of the lacZ reporter gene encoding β- galactosidase. β-Galactosidase activities of E. coli transformants harboring pOS2-lacZ were highly induced in the presence of divalent metal ion chelators such as 2,2’-dipyridyl and EDTA, and were strongly repressed in the presence of excess iron. It was also shown that the basal level β-galactosidase expression by the PentC promoter was drastically decreased by incorporating the fur gene into the expression vector. Since the newly developed iron chelator-inducible expression system is efficient and costeffective, it has wide applications in recombinant protein production.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Amann, E. / 1988 / Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli / Gene 69 : 301 ~ 315
Bagg, A. / 1987 / Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli
Baichoo, N. / 2002 / Recognition of DNA by Fur: A reinterpretation of the Fur box consensus sequence / Bacteriol 184 : 5826 ~ 5832
Baneyx, F. / 1999 / Recombinant protein expression in Escherichia coli. Curr. Opin / Biotechnol 10 : 411 ~ 421
Brickman, T. J. / 1990 / Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli / Mol. Biol 212 : 669 ~ 682
Bagg, A. / 1987 / Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli
Baichoo, N. / 2002 / Recognition of DNA by Fur: A reinterpretation of the Fur box consensus sequence / Bacteriol 184 : 5826 ~ 5832
Baneyx, F. / 1999 / Recombinant protein expression in Escherichia coli. Curr. Opin / Biotechnol 10 : 411 ~ 421
Brickman, T. J. / 1990 / Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli / Mol. Biol 212 : 669 ~ 682
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Screening of Promoters from Metagenomic DNA and Their Use for the Construction of Expression Vectors
Journal of Microbiology and Biotechnology
2008 .01
-
Construction of High Sensitive Detection System for Endocrine Disruptors with Yeast n-Alkane-assimilating Yarrowia lipolytica
Journal of Microbiology and Biotechnology
2010 .01
-
Isolation and Characterization of Paraquat-inducible Promoters from Escherichia coli
The Journal of Microbiology
1997 .12
-
Clinical Study on the Iron Absorption from Heme-Iron Polypeptide and Nonheme-Iron
Nutritional Sciences
2006 .11
-
Promoter effects of adeno-associated viral vector for transgeneexpression in the cochlea in vivo
Experimental and Molecular Medicine
2007 .01
-
Iron chelator induces MIP-3α/CCL20 in human intestinal epithelial cells: implication for triggering mucosal adaptive immunity
Experimental and Molecular Medicine
2005 .01
-
메타게놈에서 발굴한 프로모터를 장착한 새로운 항시발현 벡터의 가치평가
한국미생물·생명공학회지
2008 .01
-
Lactobacillus casei의 Phospho-β-galactosidase 유전자의 지도작성과 Escherichia coli 내에서의 발현
미생물학회지
1992 .12
-
Characterization of Promoter Sequences for Strong Expression of groEx in Escherichia coli.
The Journal of Microbiology
1996 .03
-
IscR-Dependent Gene Expression Links Iron-Sulfur Cluster Assembly to the Control of O₂-Regulated Genes in Escherichia coli
한국미생물학회 학술대회논문집
2006 .05
-
대장균에서 Superoxide 라디칼에 의하여 유도되는 프로모터의 탐색 및 특성 분석
미생물학회지
1993 .08
-
Analysis of Hot Pepper Promoters and Gene Expression Regulation
한국유전체학회 Conference
2003 .01
-
Effects of developmental iron deficiency and post-weaning iron repletion on the levels of iron transporter proteins in rats
Nutrition Research and Practice
2015 .12
-
Effects of developmental iron deficiency and post-weaning iron repletion on the levels of iron transporter proteins in rats
Nutrition Research and Practice
2015 .12
-
Enhanced Enzyme Activities of Inclusion Bodies of Recombinantβ-Galactosidase via the Addition of Inducer Analog after L-ArabinoseInduction in the araBAD Promoter System of Escherichia coli
Journal of Microbiology and Biotechnology
2008 .01
-
Roles of the GAF Domain of Enzyme INtr in Escherichia coli
한국미생물학회 학술대회논문집
2009 .05
-
NcoI Restriction Fragment Length Polymorphism of the Tumor Necrosis Factor (TNF$\alpha$) Promoter in Korean
한국동물학회 학술대회
1995 .01
-
EXPRESSION OF RECOMBINANT PROTEINS IN VARIOUS EXPRESSION SYSTEMS
한국식물학회 심포지움
1998 .01
-
High-level Expression of in vive Biotinylated Recombinant Proteins in Escherichia coli Using a New Series of pET-derived Expression Vectors
한국동물학회 학술대회
1999 .01
-
Iron Associated Heme-Derivative Biosynthesis from a Metabolic Engineered Escherichia coli
한국미생물학회 학술대회논문집
2008 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0