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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제8호
- 발행연도
- 2007.1
- 수록면
- 1,316 - 1,323 (8page)
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초록· 키워드
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In order to induce high levels of protein secretion,we have constructed a recombinant plasmid, designatedpBP244, into which was incorporated key components of thetype-II Sec-dependent secretion system, including LepB(signal peptidase), SecA (ATPase), and SecB (chaperone).The biological activities of the LepB, SecA, and SecBcomponents expressed from genes harbored by pBP244protein secretion, a pspA (Streptoccus pneumoniae surfaceprotein A) gene was cloned into pBP244, resulting inpBP438. S. typhimurium harboring pBP438 grown until thestationary phase, secreted a higher level of PspA into theculture supernatants than did the strain harboring pYA3494.The strain harboring pBP438 secreted a supernatant amountmembrane amount 1.49-fold higher than that of pYA3494.S. typhimurium χ8554 kept the Asd+ plasmid pBP244 andpBP438 for 60 generations in LB broth harboring DAP,thereby indicating that pBP244 and pBP438 were quite stablein the Salmonella strain.
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참고문헌 (26)
참고문헌 신청
/ / C. A. and A. K. Nault. 1989. Characterization of the Escherichia coli protein-export gene secB. Gene 75 : 167 ~ 175
/ / G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62 : 293 ~ 300
/ / N. J. and P. B. Wolfe. 1986. lep Operon proximal gene is not required for growth or secretion by Escherichia coli. J. Bacteriol. 166 : 83 ~ 87
A. J. M. Driessen / / and W. Wickner. 1990. ProOmpA contains secondary and tertiary structure prior to translocat
Barbosa, M. D / 2002 / Regulated expression of the Escherichia coli lepB gene as a tool for cellular testing of antimicrobial compounds that inhibit signal peptidase I in vit
/ / G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62 : 293 ~ 300
/ / N. J. and P. B. Wolfe. 1986. lep Operon proximal gene is not required for growth or secretion by Escherichia coli. J. Bacteriol. 166 : 83 ~ 87
A. J. M. Driessen / / and W. Wickner. 1990. ProOmpA contains secondary and tertiary structure prior to translocat
Barbosa, M. D / 2002 / Regulated expression of the Escherichia coli lepB gene as a tool for cellular testing of antimicrobial compounds that inhibit signal peptidase I in vit
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