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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제7호
- 발행연도
- 2007.1
- 수록면
- 1,090 - 1,097 (8page)
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초록· 키워드
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Genomic analysis of Thermococcus sp. NA1revealed the presence of a 3,927-base-pair (bp) family B-typeDNA polymerase gene, TNA1_polintein, was overexpressed in Escherichia coli, purified usingmetal affinity chromatography, and characterized. TNA1_polactivity was optimal at pH 7.5 and 75oC. TNA1_pol washighly thermostable, with a half-life of 3.5 h at 100oC and12.5 h at 95oC. Polymerase chain reaction parameters ofTNA1_pol such as eror-rate, procesivity, and extension ratewere measured in comparison with rTaq, Pfu, and KOD DNA4.45 kilobases (kb), and had a processivity of 150 nucleotides(nt) and an extension rate of 60 bases/s. Thus, TNA1_pol hasa much faster elongation rate than Pfu DNA polymerase with7-fold higher fidelity than that of rTaq.
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참고문헌 (24)
참고문헌 신청
Bae, S. S / 2006 / Thermococcus onnurineus sp. nov., a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent area at the PACMANUS field 16 : 1826 ~ 1831
Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding : 248 ~ 254
Choi, J. J / 2004 / Cloning, expression, and characterization of DNA polymerase from hyperthermophilic bacterium Aquifex pyrophilus 14 : 1022 ~ 1030
Fairfield / 1994 / On the processivity of polymerases : 118 ~ 131
Hashimoto, H / 2001 / Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 306 : 469 ~ 477
Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding : 248 ~ 254
Choi, J. J / 2004 / Cloning, expression, and characterization of DNA polymerase from hyperthermophilic bacterium Aquifex pyrophilus 14 : 1022 ~ 1030
Fairfield / 1994 / On the processivity of polymerases : 118 ~ 131
Hashimoto, H / 2001 / Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 306 : 469 ~ 477
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