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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제6호
- 발행연도
- 2007.1
- 수록면
- 1,045 - 1,048 (4page)
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Two genes, dps encoding decaprenyl diphosphatesynthase and dxs encoding 1-deoxy-D-xylulose 5-phosphatesynthase, were isolated from Rhizobium radiobacterATCC 4718. DNA sequencing analysis of the dps anddxs genes revealed an open reading frame of 1,077 bpand 1,920 bp, respectively. The heterologous expression inEscherichia coli BL21(DE3) was caried out in order toidentify their functions. Recombinant E. coli BL21(DE3)harboring the dps gene produced CoQ10CoQ8 and CoQ9, whereas E. coli harboring only thedxs gene produced more CoQ8 compared with the wild-type E. coli. Additionally, the coexpression of dps and dxsgenes in E. coli was carried out. The recombinant E. coliharboring only the dps gene produced 0.21±0.04 mg/l ofCoQ10, whereas the coexpressed E. coli with dps anddxs genes produced 0.37±0.07 mg/l of CoQ10. HPLC10 fraction (100% of thetotal CoQs distribution) was increased from 15.86±0.66%(only dps) to 29.78±1.80% (dps and dxs).
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참고문헌 (15)
참고문헌 신청
Choi, J. H / 2005 / Biotechnological production and applications of coenzyme Q10 68 : 9 ~ 15
Disch, A / 1998 / On the absence of the glyceraldehydes 3-phosphate/pyruvate pathway for isoprenoid biosynthesis in fungi and yeasts 168 : 201 ~ 208
Kawamukai, M / 2002 / Biosynthesis, bioproduction, and 94 : 511 ~ 517
Kim, S. W / 2001 / Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production 72 : 408 ~ 415
Kim. S. J / 2006 / Amplification of 1-deoxy-D-xylulose5-phosphate synthase level increases coenzyme Q10production in recombinant Escherichia coli 72 : 982 ~ 985
Disch, A / 1998 / On the absence of the glyceraldehydes 3-phosphate/pyruvate pathway for isoprenoid biosynthesis in fungi and yeasts 168 : 201 ~ 208
Kawamukai, M / 2002 / Biosynthesis, bioproduction, and 94 : 511 ~ 517
Kim, S. W / 2001 / Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production 72 : 408 ~ 415
Kim. S. J / 2006 / Amplification of 1-deoxy-D-xylulose5-phosphate synthase level increases coenzyme Q10production in recombinant Escherichia coli 72 : 982 ~ 985
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