지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 128 - 134 (7page)
이용수
초록· 키워드
오류제보하기
In order to examine efficient L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) synthesis process using whole cell biocatalyst, a thermostable L-threonine aldolase (L-TA), which cloned from Streptomyces coelicolor A3(2) and improved for stability, was expressed in a Corynebacterium glutamicum R strain. The constructed Corynebacterium expression vector, pCG-H44(1) successfully expressed L-TA in C. glutamicum R strain, but showed very low expression level. In order to improve the expression level, the expression vector named pCG-H44(2) was reconstructed by eliminating 1 nucleotide between SD sequence and start codon of LTA. The pCG-H44(2) vector plasmid was able to overexpress L-TA approximately 3.2 times higher than pCGH44(1) in C. glutamicum R strain (CGH-2). When the whole cell of CGH-2 was examined in a repeated batch system, L-threo-DOPS was successfully synthesized with a yield of 4.0 mg/ml and maintain synthesis rate constantly after 30 repeated batch reactions for 130 h.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
BAIK, SANG-HO / 2007 / Synthesis of L-threo-3,4-Dihydroxyphenylserine (L-threo-DOPS) withThermostabilized Low-Specific L-Threonine Aldolase from Streptomyces coelicolor A3(2)
Gold, L. / 1981 / Translational initiation in procaryotes / Annu. Rev. Microbiol 35 : 365 ~ 403
Goldstein, D. S. / 2006 / L-Dihydroxyphenylserine (L-DOPS): a norepinephrine prodrug / Cardiovasc. Drug Rev 24 : 189 ~ 203
Greenberg, W. A. / 2004 / Development of an efficient, scalable, aldolase-catalyzed process for enantioselective synthesis of statin intermediates / Proc. Natl. Acad. Sci. US
Hannemann, F. / 2006 / Design of an Escherichia coli system for whole cell mediated steroid synthesis and molecular evolution of steroid hydroxylases / J. Biotechnol 124 : 17
Gold, L. / 1981 / Translational initiation in procaryotes / Annu. Rev. Microbiol 35 : 365 ~ 403
Goldstein, D. S. / 2006 / L-Dihydroxyphenylserine (L-DOPS): a norepinephrine prodrug / Cardiovasc. Drug Rev 24 : 189 ~ 203
Greenberg, W. A. / 2004 / Development of an efficient, scalable, aldolase-catalyzed process for enantioselective synthesis of statin intermediates / Proc. Natl. Acad. Sci. US
Hannemann, F. / 2006 / Design of an Escherichia coli system for whole cell mediated steroid synthesis and molecular evolution of steroid hydroxylases / J. Biotechnol 124 : 17
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Synthesis of L-threo-3,4-Dihydroxyphenylserine (L-threo-DOPS) withThermostabilized Low-Specific L-Threonine Aldolase from Streptomyces coelicolor A3(2)
Journal of Microbiology and Biotechnology
2007 .01
-
Cell-Free Culture Fluid-Mediated Expression in Corynebacterium glutamicum
한국미생물학회 학술대회논문집
2010 .05
-
A Genome-Based Approach for Strain Development in Corynebacterium glutamicum
한국미생물학회 학술대회논문집
2006 .05
-
Oxidative Stress Response of Corynebacterium glutamicum
한국미생물학회 학술대회논문집
2009 .05
-
METABOLIC DESIGN IN AMINO ACID PRODUCING CORYNEBACTERIUM GLUTAMICUM
한국미생물학회 학술대회논문집
1999 .04
-
Effects of Cloned Genes on the Stability of Shuttle Vectors between Escherichia coli and Corynebacterium glutamicum
미생물학회지
1991 .07
-
Oxidative Stress Adapted Corynebacterium glutamicum
한국미생물학회 학술대회논문집
2013 .05
-
Corynebacterium glutamicum의 sigH 유전자의 분리 및 기능분석
미생물학회지
2005 .06
-
Metabolic Engineering of Corynebacterium glutamicum for High-Level L-Arginine Production
한국미생물학회 학술대회논문집
2015 .04
-
Metabolic Engineering for High-level L-Arginine Production in Corynebacterium glutamicum
한국미생물학회 학술대회논문집
2019 .04
-
Structural characterization of Tepidopterin [1-0-(L-threo-biopterin-2'-yl)- $eta$-N-acetylglucosamine] purified from Green Sulfur bacterium, Chlorobium tepidum
한국동물학회 학술대회
1994 .01
-
Isolation and Characterization of TranscriptionalElements from Corynebacterium glutamicum
Journal of Microbiology and Biotechnology
2004 .01
-
PTS-Independent Glucose Uptake Routes in Corynebacterium glutamicum
한국미생물학회 학술대회논문집
2012 .05
-
Engineering of Corynebacterium glutamicum for the Secretory Production of Recombinant Proteins
한국미생물학회 학술대회논문집
2018 .04
-
The Actinobacterium Corynebacterium glutamicum, an Industrial Workhorse
Journal of Microbiology and Biotechnology
2016 .01
-
A Cluster of Genes Involved in Arginine Biosynthesis from Corynebacterium glutamicum
한국동물학회 학술대회
1999 .01
-
Corynebacterium glutamicum에서 분리된 프로모터를 이용한 메치오닌 생합성 유전자의 조절해제
미생물학회지
2005 .12
-
Regulation of Histidine Biosynthetic Genes for Corynebacterium glutamicum
한국동물학회 학술대회
2000 .01
-
Global Transcriptome Analysis of l-lysine-producing Corynebacterium glutamicum ATCC 21300 Strain
한국미생물학회 학술대회논문집
2013 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0