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The purpose of this study was to investigate thedegradation of PVA (polyvinyl alcohol) contained in dyeingwastewater by a mixed culture of Microbacterium barkeriKCCM 10507 and Paenibacillus amylolyticus KCCM 10508.Firstly, synthetic wastewater which contained different initialconcentrations of PVA varying from 50 to 3,500 mg/l weretested to obtain optimal PVA biodegradation activity of isolatedstrains, and the above two strains were found to degrade PVAup to 90%, when the initial concentration of PVA was750 mg/l and below. Next, dyeing wastewater was tested by amixed culture of the two isolated strains, and 42% and 55% ofthe initial concentrations of PVA and COD, respectively, wasremoved after five days. MLSS was gradually increased froman initial 1,400 to 2,500 mg/l, and the pH was also increasedfrom 5.1 to 7.8. Sterilized dyeing wastewater was tested tofind the effect of strains only on the biodegradation of PVA,and PVA degradation ratio and COD removal ratio were 50%and 72.8%, respectively. Thus, the results indicated that thesetwo strains have good ability to degrade PVA and removeCOD in dyeing wastewater. Finally, it is expected that if thesetwo strains were used in the dyeing wastewater treatment,good efficiency for PVA degradation and COD removal couldbe achieved.

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