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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 175 - 186 (12page)
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Recently, reactive oxygen species (ROS) have been studied as a regulator of differentiation into specific cell types in embryonic stem cells (ESCs). However,ROS role in human ESCs (hESCs) is unknown because mouse ESCs have been used mainly for most studies.
Herein we suggest that ROS generation may play a critical role in differentiation of hESCs; ROS enhances differentiation of hESCs into bi-potent mesendodermal cell lineage via ROS-involved signaling pathways. In ROS-inducing conditions, expression of pluripotency markers (Oct4, Tra 1-60, Nanog, and Sox2) of hESCs was decreased, while expression of mesodermal and endodermal markers was increased. Moreover, these differentiation events of hESCs in ROS-inducing conditions were decreased by free radical scavenger treatment. hESC-derived embryoid bodies (EBs) also showed similar differentiation patterns by ROS induction.
In ROS-related signaling pathway, some of the MAPKs family members in hESCs were also affected by ROS induction. p38 MAPK and AKT (protein kinases B, PKB) were inactivated significantly by buthionine sulfoximine (BSO) treatment. JNK and ERK phosphorylation levels were increased at early time of BSO treatment but not at late time point. Moreover, MAPKs family-specific inhibitors could prevent the mesendodermal differentiation of hESCs by ROS induction.
Our results demonstrate that stemness and differentiation of hESCs can be regulated by environmental factors such as ROS.
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참고문헌 (23)
참고문헌 신청
Allen RG / 2000 / Oxidative stress and gene regulation / Free Radic Biol Med 28 : 463 ~ 499
Armstrong L / 2006 / The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by trans
Ateghang B / 2006 / Regulation of cardiotrophin-1 expression in mouse embryonic stem cells by HIF-1alpha and intracellular reactive oxygen species / J Cell Sci 119 : 1043 ~ 1
Buggisch M / 2007 / Stimulation of ES-cell-derived cardiomyogenesis and neonatal cardiac cell proliferation by reactive oxygen species and NADPH oxidase / J Cell Sci 120 : 88
Bunting KD / 2002 / ABC transporters as phenotypic markers and functional regulators of stem cells / Stem Cells 20 : 11 ~ 20
Armstrong L / 2006 / The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by trans
Ateghang B / 2006 / Regulation of cardiotrophin-1 expression in mouse embryonic stem cells by HIF-1alpha and intracellular reactive oxygen species / J Cell Sci 119 : 1043 ~ 1
Buggisch M / 2007 / Stimulation of ES-cell-derived cardiomyogenesis and neonatal cardiac cell proliferation by reactive oxygen species and NADPH oxidase / J Cell Sci 120 : 88
Bunting KD / 2002 / ABC transporters as phenotypic markers and functional regulators of stem cells / Stem Cells 20 : 11 ~ 20
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