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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 399 - 407 (9page)
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초록· 키워드
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Human embryonic stem cells (hESCs) need feeder cells for their maintenance in an undifferentiated state. In conventional culture systems, mouse to maintain hESCs. However, the use of MEFs elevates the risk of transmitting mouse pathogens and thus limits the potential of hESCs in cell replacement therapy. Consequently, the use of human feeder cells would be an important step forward in this in vitro technology. To address this issue, we used fibroblast-like cells differentiated from the Miz-hES6 hESC line (DiffMiz-hES6) as feeder cells to support the in vitro growth of three hESC lines. Immunofluorescence microscopy and re-verse transcription-PCR assessing the expression of undifferentiated hESC markers revealed all three hESC lines were maintained in an undifferentiated state. In vitro proliferation proceeded as efficiently as when the hESCs were cultured on MEFS. More-over, karyotype analysis revealed the chromosomal normality of the hESC lines and the DiffMiz-hES6feeders themselves after even 50 passages. Furthermore, the hESC lines maintained their pluripotency since they remained capable of forming embryoid bodies (EBs) in vitro. Thus, hESC-derived fibroblast-like cells successfully support in vitro hESC propagation.
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등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
/ / A culture system using human foreskin fibroblastsas feeder cells allows production of human embryonic stemcells blen (w e) : 1404 ~ 9
/ / A^Human feeders support prolonged undifferentiated growth ofhuman inner cell masses^embryonic stem cells : 933 ~ 6
/ / Comparative evaluation of various human feeders forprolonged undifferentiated growth of human embryonic stemcells : 546 ~ 56
/ / Contribution ofthe PI3K/Akt/PKB signal pathway to maintenance of selfrenewalin human embryonic stem cells : 534 ~ 40
/ / Feeder-free growth of undifferentiated humanembryonic stem cells i (kuma ms) : 971 ~ 4
/ / A^Human feeders support prolonged undifferentiated growth ofhuman inner cell masses^embryonic stem cells : 933 ~ 6
/ / Comparative evaluation of various human feeders forprolonged undifferentiated growth of human embryonic stemcells : 546 ~ 56
/ / Contribution ofthe PI3K/Akt/PKB signal pathway to maintenance of selfrenewalin human embryonic stem cells : 534 ~ 40
/ / Feeder-free growth of undifferentiated humanembryonic stem cells i (kuma ms) : 971 ~ 4
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