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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 71 - 77 (7page)
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The oligonucleotide microarray, a high-throughput polymorphism detection technology, holds great promise for the characterization of complex genetic variance. To achieve greater sensitivity and specificity for it to be an effective platform technology we present results and discuss some of the factors influencing signal intensities and single-mismatch discrimination in array-based mutation/SNP detection. Probes with a series of concentrations were spotted onto the slide in order to find the optimal concentration with the identifiable satisfying signals and the stable ratios between matched and mismatched probes. It was found that under our experimental conditions, when the initial probe concentration is higher than the maximum immobilization capability of the slide (7.5 µM), the hybridization signal will be saturated and the ratio between matched and mismatched probes will be more stable than at a lower probe concen-tration. Considering the cost of probes and the systematic stability, a constant spotting cone n-tration of 10 µM was selected. The stability of different types of mismatched oligo-DNA du-plexes on the glass surface was also confirmed. The results show that the order of stability of mismatched oligo-DNA duplexes on a glass surface is in general agreement with previous reports conducted using liquid and polyacryl-amide gel pads. This suggests that the influence of the mismatched base pair on the stability of the duplex in a solid hybridization system is similar to that in the solution hybridization environment.
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참고문헌 (16)
참고문헌 신청
/ / Detection of heterozygous mutations in BRCA1 using highdensity oligonucleotide arrays and two-colour fluorescenceanalysis : 441 ~ 7
/ / Dissociationkinetics of 19 base paired oligonucleotide-DNA duplexescontaining different single mismatched base pairs 15 : 797 ~ 811
/ / Hybridization of synthetic oligodeoxyribonucleotidesto phi chi 174 DNA the effect of single base pair mismatch : 3543 ~ 57
/ / Influence of nearest neighbor sequenceon the stability of base pair mismatches in long DNA;determination by temperature-gradient gel electrophoresis 21 : 5137 ~ 43
/ / Nearest-neighbor thermodynamics and NMR of DNA sequences withinternal A : 3468 ~ 77
/ / Dissociationkinetics of 19 base paired oligonucleotide-DNA duplexescontaining different single mismatched base pairs 15 : 797 ~ 811
/ / Hybridization of synthetic oligodeoxyribonucleotidesto phi chi 174 DNA the effect of single base pair mismatch : 3543 ~ 57
/ / Influence of nearest neighbor sequenceon the stability of base pair mismatches in long DNA;determination by temperature-gradient gel electrophoresis 21 : 5137 ~ 43
/ / Nearest-neighbor thermodynamics and NMR of DNA sequences withinternal A : 3468 ~ 77
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