지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 746 - 755 (10page)
이용수
초록· 키워드
오류제보하기
Although various functions of CD99 have been reported,
such as apoptosis and homotypic aggregation
of thymocyte and transendothelial migration of immune
cells, biochemical/molecular natures of CD99
are still elusive. Using mouse CD99 gene, we show that
CD99 forms homodimer through its extracellular
domain. Expression of mouse CD99 is up-regulated on
T cells after CD3-mediated activation, like the case for
human CD99. The potential of CD99 to form homodimer
was tested with a recently developed bimoleular fluorescence
complementation analysis (BiFC). In BiFC
analysis, the dimerization-induced fluorescence was
strong near the perinuclear region and was faded at the
cell membrane. However, surface expression of CD99
was still detected by flow cytometry, suggesting that
CD99 either in monomer form or in association with
other molecules exists on the cell surface. In BiFC analysis
using CD99 mutants with its extracellular, transmembrane,
or cytosolic domains changed to corresponding
human CD4 domains, the mutant replaced
with human CD4-extracellular domain did not produce
fluorescence. Purified soluble CD99-Fc fusion proteins bound to CD99-Fc immobilized onto the gold sensor
chip in surface plasmon resonance analysis, confirming
that the extracellular domain was responsible
for dimer formation. Intracytoplasmic staining for
CD99 expression in the thymocytes and mature T cells
showed that most of the cells, even the cells with low
surface level of CD99, contained the molecule inside
the cell. Our results suggest that majority of CD99 homodimers
may exit in the cell and be exported to the
cell surface, dissociating from each other, after a certain
regulatory signal is delivered.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Ambros IM / 1886-93 / MIC2 is a specific marker for Ewing's sarcoma and peripheral primitive neuroectodermal tumors Evidence for a common histogenesis of Ewing's sarcoma and
Aparicio F / / In vitro and in vivo mapping of the Prunus necrotic ringspot virus coat protein C-terminal dimerization domain by bimolecular fluorescence complementation : 17
Bernard G / eurjimmunol2000;30 / up-regulates alpha4-beta1-dependent T cell adhesion to inflamed vascular endo-thelium under flow conditions : 3061 ~ 5
Bernard G / jimmunol1995;154jimmunol1997;158 / specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes Apoptosis of immature thymocytes mediated by E2/CD99 : 26 2
Bernard G / oncogene2004;23 / Molecular mechanisms of CD99-induced caspase-independent cell death and cell-cell adhesion in Ewing's sarcoma cells actin and zyxin as key intra
Aparicio F / / In vitro and in vivo mapping of the Prunus necrotic ringspot virus coat protein C-terminal dimerization domain by bimolecular fluorescence complementation : 17
Bernard G / eurjimmunol2000;30 / up-regulates alpha4-beta1-dependent T cell adhesion to inflamed vascular endo-thelium under flow conditions : 3061 ~ 5
Bernard G / jimmunol1995;154jimmunol1997;158 / specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes Apoptosis of immature thymocytes mediated by E2/CD99 : 26 2
Bernard G / oncogene2004;23 / Molecular mechanisms of CD99-induced caspase-independent cell death and cell-cell adhesion in Ewing's sarcoma cells actin and zyxin as key intra
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
사람의 결핵균 특이 CD3+CD4-CD8-γδ T림프구 클론의 항원인지 과정에서 CD1의 역할
The Journal of the Korean Society for Microbiology
1993 .12
-
Abscisic acid가 Cd2+에 의한 닭의장풀의 생리적 반응에 미치는 영향
환경생물
2005 .01
-
Isolation of CD4 Genomic Clones and Role of Its 5' Upstream Region in CD4 Expression
미생물학회지
1992 .12
-
Antisense Oligonucleotides against p56$^{lck}$ Block the Differentiation of Immature CD4$^{+}$CD8$^{+}$ Thymocytes to Mature CD4$^{+}$ or CD8$^{+}$ Thymocytes
한국동물학회 학술대회
1996 .01
-
CdS 분말 및 박막의 광학적 특성
한국결정학회지
2008 .01
-
CD43 cross-linking increases the Fas-induced apoptosis through induction of Fas aggregation in Jurkat T-cells
Experimental and Molecular Medicine
2006 .01
-
Expression of CD30 in Testis and Epididymis of Adult Mice
Korean journal of biological sciences
2004 .01
-
CD99 activates T cells via a costimulatory function thatpromotes raft association of TCR complex and tyrosinephosphorylation of TCR ζ
Experimental and Molecular Medicine
2007 .01
-
Concurrent Monitoring of Actin Cytoskeleton CD3, CD4, and CD8 for Early Stage High-Content HIV Diagnosis
한국분석과학회 학술대회
2014 .06
-
Intracellular CD154 Expression Reflects Antigen-specific CD8+ T Cells butShows Less Sensitivity than Intracellular Cytokine and MHC TetramerStaining
Journal of Microbiology and Biotechnology
2007 .01
-
Activation of B cells using Schneider 2 cells expressing CD40 ligand for the enhancement of antigen presentation in vitro
Experimental and Molecular Medicine
2005 .01
-
$CdS/CuInSe_2$태양전지의 Window Layer로 쓰이는 CdS박막의 진공증착법에 따른 전기적.광학적 성질
한국결정학회지
1997 .01
-
Sorting CD4⁺ T Cells in Blood by Magnetic Nanoparticles Coated with Anti-CD4 Antibody
한국자기학회 학술연구발표회 논문개요집
2007 .05
-
CD137 induces adhesion and cytokine production in human monocytic THP-1cells
Experimental and Molecular Medicine
2005 .01
-
Proteomic Analysis of Resting and Activated Human CD8+ T Cells
Journal of Microbiology and Biotechnology
2006 .01
-
이자율모형과 몬테칼로시뮬레이션을 이용한 CD금리 분포 추정
Journal of The Korean Data Analysis Society
2011 .01
-
OKT3 투여후 말초혈내 림프구수 및 림프구아형(CD3, CD4, CD8, CD19, CD16+56)수의 변화에 관한 면역학적 연구
대한임상검사과학회지
1998 .01
-
키토산올리고당이 카드뮴을 투여한 생쥐의 간독성에 미치는 효과
한국현미경학회지
2003 .01
-
HIV 감염자의 혈청내 CD4 Antigen과 Soluble CD8치
The Journal of the Korean Society for Microbiology
1991 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0