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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.1
- 수록면
- 310 - 316 (7page)
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초록· 키워드
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We have used a surface plasmon resonance bio-sensor (SPR, BIACORE 20) to detect antibodies synovial fluids of rheumatoid arthritis (RA) and os-teoarthritis (OA). Recombinant human GPI proteins fused with or without NusA were expressed in E. coli, purified to homogeneity and immobilized in flow cels of CM5 sensor chips. The flow cells im-mobilized with NusA protein or bovine serum al-bumin were used to monitor non-specific binding. Synovial fluid samples from RA patients showed a significantly higher level of binding to recombi-nant GPI proteins than samples from OA patients. teins were confirmed to be imunoglobulin through the administration of anti-human imunoglobulin. NusA fusion protein was excelent for this assay because of a low background binding activity in the SPR analysis and its advantage of increased solubility in recombinant protein production. These results sugested a useful utilization of recombi-nant NusA-GPI fusion protein for the detection of autoantibodies against GPI in RA patients.
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