지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제5호
- 발행연도
- 2012.1
- 수록면
- 654 - 658 (5page)
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초록· 키워드
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Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA.
The efficient and reliable preparation of SF proteomes is a critical step towards biomarker discovery. In this study,we have optimized a pretreatment method for twodimensional gel electrophoresis (2DE) separation of the SF proteome, by enriching low-abundance proteins and simultaneously removing hyaluronic acid, albumin, and IgG. SF samples pretreated using this optimized method were then evaluated by 1DE and 2DE separation followed by immunodetection of cartilage oligomeric matrix protein (COMP), a known OA biomarker, and by the identification of 3 proteins (apolipoprotein, haptoglobin precursor, and fibrinogen D fragment) that are related to joint diseases.
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참고문헌 (17)
참고문헌 신청
Chang, X. / 2010 / Increased expression of carbonic anhydrase I in the synovium of patients with ankylosing spondylitis / BMC Musculoskelet. Disord 11 : 279 ~
De Ceuninck, F. / 2009 / Proteomics: Addressing the challenges of osteoarthritis / Drug Discov. Today 14 : 661 ~ 667
Ghosh, P. / 1994 / The role of hyaluronic acid (hyaluronan) in health and disease: Interactions with cells, cartilage and components of synovial fluid / Clin. Exp. Rheumat 12
Gibson, D. S. / 2007 / The human synovial fluid proteome: A key factor in the pathology of joint disease / Proteomics Clin. Appl 1 : 889 ~ 899
Kamphorst, J. J. / 2007 / Profiling of endogenous peptides in human synovial fluid by NanoLC-MS: Method validation and peptide identification / J. Proteome Res 6 : 4388 ~ 439
De Ceuninck, F. / 2009 / Proteomics: Addressing the challenges of osteoarthritis / Drug Discov. Today 14 : 661 ~ 667
Ghosh, P. / 1994 / The role of hyaluronic acid (hyaluronan) in health and disease: Interactions with cells, cartilage and components of synovial fluid / Clin. Exp. Rheumat 12
Gibson, D. S. / 2007 / The human synovial fluid proteome: A key factor in the pathology of joint disease / Proteomics Clin. Appl 1 : 889 ~ 899
Kamphorst, J. J. / 2007 / Profiling of endogenous peptides in human synovial fluid by NanoLC-MS: Method validation and peptide identification / J. Proteome Res 6 : 4388 ~ 439
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