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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1 - 12 (12page)
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B lymphocytes are produced from hematopoietic stem cells (HSCs) through the highly ordered process of B lymphopoiesis, which is regulated by a complex network of cytokines, chemokines and cell adhesion molecules derived from the hematopoietic niche.
Primary osteoblasts function as an osteoblastic niche (OBN) that supports in vitro B lymphopoiesis. However, there are significant limitations to the use of primary osteoblasts, including their relative scarcity and the consistency and efficiency of the limited purification and proliferation of these cells. Thus, development of a stable osteoblast cell line that can function as a biomimetic or artificial OBN is necessary. In this study, we developed a stable osteoblastic cell line, designated OBN4, which functions as an osteoblast-based artificial niche that supports in vitro B lymphopoiesis. We demonstrated that the production of a B220+ cell population from Lineage− (Lin−) Sca-1+ c-Kit+ hematopoietic stem and progenitor cells (HSPCs) was increased ~ 1.7-fold by OBN4 cells relative to production by primary osteoblasts and OP9 cells in coculture experiments. Consistently, OBN4 cells exhibited the highest production of B220+ IgM+ cell populations (6.7±0.6–13.6±0.6%) in an IL-7- and stromal cell-derived factor 1-dependent manner, with higher production than primary osteoblasts (3.7±0.5–6.4±0.6%) and OP9 cells (1.8±0.6–3.9±0.5%). In addition, the production of B220+ IgM+ IgD+ cell populations was significantly enhanced by OBN4 cells (15.4±1.1–18.9±3.2%) relative to production by primary osteoblasts (9.5±0.6–14.6±1.6%) and OP9 cells (9.1±0.5– 10.3±1.8%). We conclude that OBN4 cells support in vitro B lymphopoiesis of Lin− Sca-1+ c-Kit+ HSPCs more efficiently than primary osteoblasts or OP9 stromal cells.
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참고문헌 (24)
참고문헌 신청
Aguila JR / 2011 / SALL4 is a robust stimulator for the expansion of hematopoietic stem cells / Blood 118 : 576 ~ 585
Calvi LM / 2013 / Cellular complexity of the bone marrow hematopoietic stem cell niche / Calcif Tissue Int 94 : 112 ~ 124
Carpenter L / 2011 / Human induced pluripotent stem cells are capable of B-cell lymphopoiesis / Blood 117 : 4008 ~ 4011
Cho SK / 1999 / Functional characterization of B lymphocytes generated in vitro from embryonic stem cells / Proc Natl Acad Sci USA 96 : 9797 ~ 9802
Dzierzak E / 2008 / Of lineage and legacy : the development of mammalian hematopoietic stem cells / Nat Immunol 9 : 129 ~ 136
Calvi LM / 2013 / Cellular complexity of the bone marrow hematopoietic stem cell niche / Calcif Tissue Int 94 : 112 ~ 124
Carpenter L / 2011 / Human induced pluripotent stem cells are capable of B-cell lymphopoiesis / Blood 117 : 4008 ~ 4011
Cho SK / 1999 / Functional characterization of B lymphocytes generated in vitro from embryonic stem cells / Proc Natl Acad Sci USA 96 : 9797 ~ 9802
Dzierzak E / 2008 / Of lineage and legacy : the development of mammalian hematopoietic stem cells / Nat Immunol 9 : 129 ~ 136
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