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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
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- 학술저널
- 저자정보
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- 2015.1
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- 1 - 7 (7page)
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Pulmonary dysfunction caused by ischemia-reperfusion injury is the leading cause of mortality in lung transplantation. We aimed to investigate the effects of sevoflurane pretreatment on lung permeability, tight junction protein occludin and zona occludens 1 (ZO-1) expression, and translocation of protein kinase C (PKC)-α after ischemia–reperfusion. A lung ischemia-reperfusion injury model was established in 96 male Wistar rats following the modified Eppinger method. The rats were divided into four groups with 24 rats in each group: a control (group C), an ischemia-reperfusion group (IR group), a sevoflurane control group (sev-C group), and a sevoflurane ischemia-reperfusion group (sev–IR group). There were three time points in each group: ischemic occlusion for 45 min, reperfusion for 60 min and reperfusion for 120 min; and there were six rats per time point. For the 120-min reperfusion group, six extra rats underwent bronchoalveolar lavage. Mean arterial pressure (MAP) and pulse oxygen saturation (SpO2) were recorded at each time point. The wet/dry weight ratio and lung permeability index (LPI) were measured.
Quantitative RT-PCR and Western blot were used to measure pulmonary occludin and ZO-1, and Western blot was used to measure cytosolic and membranous PKC-α in the lung. Lung permeability was significantly increased after ischemia–reperfusion.
Sevoflurane pretreatment promoted pulmonary expression of occludin and ZO-1 after reperfusion and inhibited the translocation of PKC-α. In conclusion, sevoflurane pretreatment alleviated lung permeability by upregulating occludin and ZO-1 after ischemia–reperfusion. Sevoflurane pretreatment inhibited the translocation and activation of PKC-α, which also contributed to the lung-protective effect of sevoflurane.
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참고문헌 (20)
참고문헌 신청
Aijaz S / 2006 / Tight junctions : Molecular architecture and function / Int Rev Cytol 248 : 261 ~ 298
Anderson JM / 1995 / Tight junctions and the molecular basis for regulation of paracellular permeability / Am J Physiol 269 : G467 ~ G475
Ando-Akatsuka Y / 1996 / Interspecies diversity of the occluding sequence: cDNA cloning of human, mouse, dog and rat kangaroo homologues / J Cell Biol 133 : 43 ~ 47
Andreeva AY / 2001 / Protein kinase C regulates the phosphorylation and cellular localization of occludin / J Biol Chem 276 : 38480 ~ 38486
Bellido-Reyes YA / 2006 / Cytosolic phospholipase A2 inhibition attenuates ischemia?reperfusion injury in an isolated rat lung model / Transplantation 81 : 1700 ~ 1707
Anderson JM / 1995 / Tight junctions and the molecular basis for regulation of paracellular permeability / Am J Physiol 269 : G467 ~ G475
Ando-Akatsuka Y / 1996 / Interspecies diversity of the occluding sequence: cDNA cloning of human, mouse, dog and rat kangaroo homologues / J Cell Biol 133 : 43 ~ 47
Andreeva AY / 2001 / Protein kinase C regulates the phosphorylation and cellular localization of occludin / J Biol Chem 276 : 38480 ~ 38486
Bellido-Reyes YA / 2006 / Cytosolic phospholipase A2 inhibition attenuates ischemia?reperfusion injury in an isolated rat lung model / Transplantation 81 : 1700 ~ 1707
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