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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 116 - 121 (6page)
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Influenza vaccine strains have been traditionally developed by annual reassortment between vaccine donor strain and the epidemic virulent strains. The classical method requires screening and genotyping of the vaccine strain among various reassortant viruses, which are usually laborious and time-consuming. Here we developed an efficient reverse genetic system to generate the 6:2 reassortant vaccine virus from cDNAs derived from the influenza RNAs. Thus, cDNAs of the two RNAs coding for surface antigens, haemagglutinin and neuraminidase from the epidemic virus and the 6internal genes from the donor strain were transfected into cells and the infectious viruses of 6:2 defined RNA ratio were rescued. X-31 virus (a high- growth virus in embryonated eggs) and its cold-adapted strain X-31 ca were judiciously chosen as donor strains for the generation of inactivated vaccine and live-attenuated vaccine,respectively. The growth properties of these recombinant viruses in embryonated chicken eggs and MDCK cell were indistinguishable as compared to those generated by classical reassortment process.
Based on the reverse genetic system, we generated 6+ 2 reassortant avian influenza vaccine strains corresponding to the A/Chicken/Korea/ MS96 (H9N2) and A/Indonesia/5/2005 (H5N1). The results would serve as technical platform for the generation of both injectable inactivated vaccine and the nasal spray live attenuated vaccine for the prevention of influenza epidemics and pandemics.
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참고문헌 (20)
참고문헌 신청
Bardiya N / 2005 / Influenza vaccines: recent advances in production technologies / Applied Microbiology and Biotechnology 67 : 299 ~ 305
Cinatl J / 2007 / The threat of avian influenza A (H5N1). Part I: epidemiologic concerns and virulence determinants / Medical Microbiology and Immunology 196 : 181 ~ 190
Cox RJ / 2004 / Influenza Virus: Immunity and Vaccination Strategies. Comparison of theImmune Response to Inactivated and Live, Attenuated Influenza Vaccines / Scand J Immuno
Ha SH / 2006 / A multiplex RT-PCR method for screening of reassortant live influenza vaccine virus strains / J Virol Methods 134 : 154 ~ 163
Hoffmann E / 2000 / "Ambisense" Approach for the Generation of Influenza A Virus: vRNA and mRNA Synthesis from One Template / Virology 267 : 310 ~ 317
Cinatl J / 2007 / The threat of avian influenza A (H5N1). Part I: epidemiologic concerns and virulence determinants / Medical Microbiology and Immunology 196 : 181 ~ 190
Cox RJ / 2004 / Influenza Virus: Immunity and Vaccination Strategies. Comparison of theImmune Response to Inactivated and Live, Attenuated Influenza Vaccines / Scand J Immuno
Ha SH / 2006 / A multiplex RT-PCR method for screening of reassortant live influenza vaccine virus strains / J Virol Methods 134 : 154 ~ 163
Hoffmann E / 2000 / "Ambisense" Approach for the Generation of Influenza A Virus: vRNA and mRNA Synthesis from One Template / Virology 267 : 310 ~ 317
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