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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 208 - 219 (12page)
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Kaempferol is the major flavonol in green tea and exhibits many biomedically useful properties such as antioxidative, cytoprotective and anti-apoptotic activities.
To elucidate its effects on the skin, we investigated the transcriptional profiles of kaempferol-treated HaCaT cells using cDNA microarray analysis and identified 147 transcripts that exhibited significant changes in expression. Of these, 18 were up-regulated and 129 were down-regulated. These transcripts were then classified into 12 categories according to their functional roles: cell adhesion/cytoskeleton, cell cycle, redox homeostasis, immune/defense responses, metabolism, protein biosynthesis/modification, intracellular transport, RNA processing, DNA modification/ replication, regulation of transcription, signal transduction and transport. We then analyzed the promoter sequences of differentially-regulated genes and identified over-represented regulatory sites and candidate transcription factors (TFs) for gene regulation by kaempferol. These included c-REL, SAP-1, Ahr-ARNT, Nrf-2, Elk-1, SPI-B, NF-κB and p65. In addition, we validated the microarray results and promoter analyses using conventional methods such as real-time PCR and ELISA-based transcription factor assay. Our microarray analysis has provided useful information for determining the genetic regulatory network affected by kaempferol, and this approach will be useful for elucidating gene-phytochemical interactions.
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참고문헌 (23)
참고문헌 신청
Ahmad N / 1999 / Green tea polyphenols and cancer: Biologic mechanisms and practical implications / Nutr Rev 57 : 78 ~ 83
Ahmad N / 2001 / Cutaneous photochemoprotection by green tea: A brief review / Skin Pharmacol Appl Skin Physiol 14 : 69 ~ 76
Ahn JI / 2004 / Comprehensive transcriptome analysis of differentiation of embryonic stem cells into midbrain and hindbrain neurons / Dev Bio 265 : 491 ~ 501
Alizadeh AA / 2000 / Distinct types of diffuse large B-cell-lymphoma identified by gene expression profiling / Nature 403 : 503 ~ 11
Ashburner M / 2000 / Gene ontology: tool for the unification of biology / Nat Genet 25 : 25 ~ 9
Ahmad N / 2001 / Cutaneous photochemoprotection by green tea: A brief review / Skin Pharmacol Appl Skin Physiol 14 : 69 ~ 76
Ahn JI / 2004 / Comprehensive transcriptome analysis of differentiation of embryonic stem cells into midbrain and hindbrain neurons / Dev Bio 265 : 491 ~ 501
Alizadeh AA / 2000 / Distinct types of diffuse large B-cell-lymphoma identified by gene expression profiling / Nature 403 : 503 ~ 11
Ashburner M / 2000 / Gene ontology: tool for the unification of biology / Nat Genet 25 : 25 ~ 9
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