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Subject

Protective Effects of α-Mangostin on UVB-induced Oxidative Stress and Cellular Senescence
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인간각질형성세포에서 알파망고스틴의 자외선B 의존적 산화적스트레스에 대한 세포보호 효과

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Type
Academic journal
Author
Journal
한국피부과학연구원 아시안뷰티화장품학술지 아시안뷰티화장품학술지 제13권 제6호 KCI Accredited Journals
Published
2015.1
Pages
813 - 818 (6page)

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Protective Effects of α-Mangostin on UVB-induced Oxidative Stress and Cellular Senescence
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Abstract· Keywords

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One of subtypes in ultraviolet (UV), UVB, has been reported that the most powerful factors causing photoaging and DNA lesions. Finding and developing both cost-effective and efficient materials are the urgent issues to protect the skin from UVB-induced damages. Alpha-mangostin (α-mangostin), the first xanthone isolated from Garcinia mangostana (mangosteen), has been studied for anti-inflammation and antioxidant properties. The purpose of this study to investigate effects of α-mangostin in HaCaT, keratinocyte against UVB radiation. To evaluate effects of α-mangostin upon UVB-induced cytotoxic damages, watersoluble tetrazolium salt (WST-1) assay, flow Cytometry analysis, qunatitative real-time PCR (qRT-PCR), and senescence associated β-galactosidase assay (SA-β-gal assay) were performed. Pretreatment of α-mangostin was verified protective effects on UVB-induced proliferative restrain, apoptotic cell death and senescence, in HaCaT keratinocytes. These results indicated the protective effects of α-mangostin against UVB, and suggest as a cosmeceutical ingredient.

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