지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 22 - 31 (10page)
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초록· 키워드
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Purpose : Bronchopulmonary dysplasia (BPD) is characterized by arrested vascular and
alveolar growth in the premature lung. Considering the consequences of arrested lung growth,
the idea of administering bone marrow cells to enhance the inborn repair mechanism is
promising as this may reduce the morbidity and mortality of BPD. We followed enhanced
green fluorescent protein (EGFP)-labeled bone marrow cells (BMC) injected intraperitoneally
into non-EGFP mice in order to determine their fate after transplantation.
Methods : An angiogenesis inhibitor, SU1498, was injected subcutaneously on day 3 in
non-EGFP C57BL/6 newborn mice to create a model of arrested alveolar development. On
the following day, 1×106 BMCs isolated from major histocompatibility complex (MHC)-
matched syngenic EGFP mice were injected intraperitoneally to non-EGFP BPD mice. Morphometric
analysis, immunostaining, and confocal microscopy were performed to determine
the fate of EGFP-positive stem cells in the injured lung.
Results : SU1498 injection reduced alveolar surface area and mean alveolar volume in
newborn mice. BMC injection resulted in recovery of lung structure comparable to controls.
EGFP-positive BMCs were identified in the lungs of the recipient mice after intraperitoneal
injection. The injected EGFP cells were co-stained with endothelial and epithelial cells of
the developing lung as determined by confocal microscopy.
Conclusion : Our results illustrated that EGFP-positive BMCs engrafted and transdifferentiated
into epithelial and endothelial cells after intraperitoneal injection in a mouse
model of arrested alveolar development.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Abman SH / 2001 / Bronchopulmonary dysplasia: "a vascular hypothesis" / Am J Respir Crit Care Med 164 : 1755 ~ 1756
Amy RW / 1977 / Thurlbeck WM. Postnatal growth of the mouse lung / J Anat 124 : 131 ~ 151
Balasubramaniam V / 2007 / Balasubramaniam V, Mervis CF, Maxey AM, Markham NE, Abman SH. Hyperoxia reduces bone marrow, circulating, and lung endothelialprogenitor cells in
Beckstead JH / 1994 / A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues / J Histochem Cytochem 42 : 1127 ~ 1134
Cho SJ / 2005 / Retinoic acid and erythropoietin maintain alveolar development in mice treated with an angiogenesis inhibitor / Am J Respir Cell Mol Biol 33 : 622 ~ 628
Amy RW / 1977 / Thurlbeck WM. Postnatal growth of the mouse lung / J Anat 124 : 131 ~ 151
Balasubramaniam V / 2007 / Balasubramaniam V, Mervis CF, Maxey AM, Markham NE, Abman SH. Hyperoxia reduces bone marrow, circulating, and lung endothelialprogenitor cells in
Beckstead JH / 1994 / A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues / J Histochem Cytochem 42 : 1127 ~ 1134
Cho SJ / 2005 / Retinoic acid and erythropoietin maintain alveolar development in mice treated with an angiogenesis inhibitor / Am J Respir Cell Mol Biol 33 : 622 ~ 628
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