지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 49 - 60 (12page)
이용수
초록· 키워드
오류제보하기
Background : Anticancer effects of herbal medicine have been reported in various types of cancer, but the systematic approaches to explain molecular mechanism(s) are not established yet.
Objective : The purpose of this study is to investigate the apoptotic cell death by Egg White combined Chalcanthite in NCI-H460 human lung cancer cells.
Methods : Inhibitory effects were estimated by the MTT-assay. Cancer cells were stained with DAPI and showed condensed and fragmented nuclei. The expression of cleaved caspase-3, bcl-2, and bax was detected by western blotting. To establish a basis of understanding for anti-cancer mechanism, whole proteins have been obtained from NCI-H460 harvested at 24 hrs after the treatment of Egg White combined Chalcanthite, protein expression has been profiled by 2DE-based proteomic approach.
Results : NCI-H460 human lung cancer cells were treated by three samples of IS3, IS4 and IS5. IS4 inhibited most effectively the growth of NCI-H460 human lung cancer cells. The expression of cleaved caspase-3 increased in IS4 in a concentration-dependent manner. Various changes of the protein expression have been monitored, and most frequent dysregulation was found in Vimentin, Lamin-A/C.
Conclusion : Egg White combined-Chacanthite inhibited the growth of NCI-H460 human lung cancer cells by inducing the apoptotic cell death via caspase-3 activation. Based upon the present findings, the further study will focus on monitoring various cancer survival factors after artificial regulation of the proteins identified, and it would be the basis for the understanding of the Chacabthite anti-cancer effect(s) at the molecular level.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
/ / http://kangtai-2000.diytrade.com
Adams JM / 1998 / The Bcl-2 protein family: arbiters of cell survival / Science. 281 (5381) : 1322 ~ 1326
Chang HY / 2000 / Proteases for cell suicide: Functions and regulations of caspases / Microbiol Mol Biol Rev. 64 : 821 ~ 846
Choi HD / 2002 / Functional Properties of Phosvitin and Lysozyme as Food Material / Administry of Food and Agriculture & Forestry : 90 ~ 92
Evans VG / 1993 / Multiple pathways to apoptosis / Cell Biol Int. 17 : 461 ~ 476
Chang HY / 2000 / Proteases for cell suicide: Functions and regulations of caspases / Microbiol Mol Biol Rev. 64 : 821 ~ 846
Choi HD / 2002 / Functional Properties of Phosvitin and Lysozyme as Food Material / Administry of Food and Agriculture & Forestry : 90 ~ 92
Evans VG / 1993 / Multiple pathways to apoptosis / Cell Biol Int. 17 : 461 ~ 476
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Inhibitory Effect of Bee Venom Toxin on Lung Cancer NCI H460 Cells Growth Through Induction of Apoptosis via Death Receptor Expressions
대한침구의학회지
2014 .01
-
사람 폐암세포주에서의 bcl-2 안티센스 처리에 의한 효과
Toxicological Research
2002 .12
-
NCI-H460 폐암 유발 누드마우스 모형을 이용한 난담반의 항암 효과 연구
동의생리병리학회지
2010 .04
-
The Incidence Rate and Severity of Orthotopic Lung Cancer in an Animal Model Depends on the Number of A549 Cells and Transplantation Period
Laboratory Animal Research
2010 .12
-
비소세포성폐암에 대한 자연살해세포의 항암효능
약학회지
2011 .01
-
폐암 세포에서 기저 상태와 TNF-${\alpha}$ 자극 시 NF-${\kappa}B$의 활성화
Tuberculosis and Respiratory Diseases
2002 .01
-
Proteomic Profiling of Human Small Cell Lung Cancer Cell Line NCI-H211
Cancer Research and Treatment
2003 .01
-
인체 폐암종에서 p53의 발현에 관한 연구
Tuberculosis and Respiratory Diseases
1993 .01
-
비소세포 폐암에서의 p53 단백의 발현 양상
Tuberculosis and Respiratory Diseases
1993 .01
-
Anticancer effect of mountain ginseng Pharmacopuncture to the nude mouse of lung carcinoma induced by NCI-H460 human non-small cell lung cancer cells
Journal of Pharmacopuncture
2010 .01
-
Antitumor Effect of Hang-Am-Dan Non-boiled Water Extracts on NCI-H460 Tumor Regression Model
대한한의학회지
2010 .05
-
인체폐암세포 NCI-H460 및 A549의 apoptosis 유발에 미치는 參_補肺湯의 영향
대한한방내과학회지
2007 .01
-
Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB
Biomolecules & Therapeutics(구 응용약물학회지)
2014 .01
-
NCI-H460 폐암 유발 누드마우스 모형을 이용한 난담반의 항암 효과 연구
동의생리병리학회지
2010 .01
-
Lung Cancer in Korea
Cancer Research and Treatment
2002 .01
-
NCI-H157 폐암 세포주에서 Caspase Cascade 활성을 통한 Arsenic Trioxide와 Sulindac 병합요법의 세포고사효과
Tuberculosis and Respiratory Diseases
2004 .01
-
비소화합물에 의한 A549 폐암세포의 증식억제에 관한 연구
동의생리병리학회지
2005 .01
-
유방암 줄기세포 개념 및 제한점
의생명과학
2018 .01
-
Epidemiology of Lung Cancer in Korea: Recent Trends
Tuberculosis and Respiratory Diseases
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0