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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 104 - 110 (7page)
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초록· 키워드
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Purpose: The extraction of nucleic acid is initially a limiting step for successful molecular-based diagnostic workup.
This study aims to compare the effectiveness of three automated DNA extraction systems for clinical laboratory use. Materials and Methods: Venous blood samples from 22 healthy volunteers were analyzed using QIAamp®Blood Mini Kit (Qiagen), MagNA Pure LC Nucleic Acid Isolation Kit I (Roche), and Magtration-Magnazorb DNA common kit-200N (PSS). The concentration of extracted DNAs was measured by NanoDrop ND-1000 (PeqLab).
Also, extracted DNAs were confirmed by applying in direct agarose gel electrophoresis and were amplified by polymerase chain reaction (PCR) for human beta-globin gene. Results: The corrected concentrations of extracted DNAs were 25.42 ± 8.82 ng/μL (13.49-52.85 ng/μL) by QIAamp® Blood Mini Kit (Qiagen), and 22.65 ± 14.49ng/μL (19.18-93.39 ng/μL) by MagNA Pure LC Nucleic Acid Isolation Kit I, and 22.35 ± 6.47 ng/μL (12.57-35.08ng/μL) by Magtration-Magnazorb DNA common kit-200N (PSS). No statistically significant difference was noticed among the three commercial kits (p > 0.05). Only the mean value of DNA purity through PSS was slightly lower than others. All the extracted DNAs were successfully identified in direct agarose gel electrophoresis. And all the product of beta-globin gene PCR showed a reproducible pattern of bands. Conclusion: The effectiveness of the three automated extraction systems is of an equivalent level and good enough to produce reasonable results. Each laboratory could select the automated system according to its clinical and laboratory conditions.
목차
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참고문헌 (18)
참고문헌 신청
Beuselinck K / 2005 / Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR / J Clin Microbiol 43 : 5541 ~ 5546
Bogner PN / 2006 / Extraction of nucleic acids. In Molecular diagnostics for the clinical laboratorian. 2nd ed. / Humana Press : 25 ~ 30
Edvinsson B / 2004 / DNA extraction and PCR assays for detection of Toxoplasma gondii / APMIS 112 : 342 ~ 348
Garg UC / 1996 / Simple and rapid method for extraction of DNA from fresh and cryopreserved clotted human blood / Clin Chem 42 : 647 ~ 648
Gustafson S / 1987 / Parameters affecting the yield of DNA from human blood / Anal Biochem 165 : 294 ~ 299
Bogner PN / 2006 / Extraction of nucleic acids. In Molecular diagnostics for the clinical laboratorian. 2nd ed. / Humana Press : 25 ~ 30
Edvinsson B / 2004 / DNA extraction and PCR assays for detection of Toxoplasma gondii / APMIS 112 : 342 ~ 348
Garg UC / 1996 / Simple and rapid method for extraction of DNA from fresh and cryopreserved clotted human blood / Clin Chem 42 : 647 ~ 648
Gustafson S / 1987 / Parameters affecting the yield of DNA from human blood / Anal Biochem 165 : 294 ~ 299
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