지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 292 - 297 (6page)
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초록· 키워드
오류제보하기
DNA extraction is a prerequisite for the identification of pathogens in clinical samples. Commercial DNA extractionkits generally involve time-consuming and laborious multi-step procedures.
In the present study, our modified DNA isolation method for saliva samples allows for the quick detection ofpathogens associated with dental caries or periodontitis by PCR within 1 h. To release DNA from the bacteria, 1min of boiling was adequate, and the resulting isolated DNA can be used many times and is suitable for longterm storage of at least 13 months at 4℃, and even longer at -20℃.
In conclusion, our modified DNA extraction method is simple, rapid, and cost-effective, and suitable forpreparing DNA from clinical samples for PCR for the rapid detection of oral pathogens from saliva.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
/ / An investigation of a rapid DNA extraction method for routine MAS in the S.A. Barley Improvement Program
Eisenach KD / 1991 / Detection of Mycobacterium tuberculosis in clinical sputum samples using a polymerase chain reaction / Am Rev Respir Dis 144 : 1160 ~ 1163
Lin CY / 1994 / Rapid and specific detection of the leukotoxin sequences of Actinobacillus actinomycetemcomitans from periodontal pockets by the polymerase chain reaction / J
Okada M / 2000 / Detection of Actinobacillus actinomycetemcomitans and porphyromonas gingivalis in dental plaque samples from children 2 to 12 years of age / J Clin Periodont
Okada M / 2002 / PCR detection of Streptococcus mutans and S. sobrinus in dental plaque samples from Japanese pre-school children / J Med Mcrobiol 51 : 443 ~ 447
Eisenach KD / 1991 / Detection of Mycobacterium tuberculosis in clinical sputum samples using a polymerase chain reaction / Am Rev Respir Dis 144 : 1160 ~ 1163
Lin CY / 1994 / Rapid and specific detection of the leukotoxin sequences of Actinobacillus actinomycetemcomitans from periodontal pockets by the polymerase chain reaction / J
Okada M / 2000 / Detection of Actinobacillus actinomycetemcomitans and porphyromonas gingivalis in dental plaque samples from children 2 to 12 years of age / J Clin Periodont
Okada M / 2002 / PCR detection of Streptococcus mutans and S. sobrinus in dental plaque samples from Japanese pre-school children / J Med Mcrobiol 51 : 443 ~ 447
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