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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.1
- 수록면
- 132 - 139 (8page)
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Purpose: The aim of this study was to know whether and how tamsulosin induces apoptosis of normal rat
prostate cells, and the relationship between apoptosis and clusterin expression.
Materials and Methods: We used a prostate cell line, NRP-152 cells which are the basal epithelium cell
originated from rat prostate. The NRP-152 cells were treated with various concentrations (50, 100, 200, 400 uM)
of tamsulosin for 24 h. To evaluate apoptosis, the cultured NRP-152 cells were stained with Heochst 33258
and Propidium Iodide (PI) without fixation. We also examined DNA fragmentation analysis to confirm apoptosis.
In addition, to elucidate the signal transduction pathway by which apoptosis is induced, we examined Bcl-2 family
proteins such as Bcl-2, Bax, Bad, Bcl-xL, and Bim by real-time RT-PCR.
Results: After tamsulosin treatment, the rate of apoptosis was 25% at 100μM, 50% at 200μM, and 63%
at 400μM, whereas the rate of necrosis was 10% at 100μM, 38% at 200μM, and 56% at 400μM. DNA
fragmentation was also gradually increased and the highest at 400μM, similar to apoptotic cell rates. As a result
of real-time RT-PCR, there was significant difference of Bcl-2 and Bim mRNA expression among the groups.
Expression of clusterin protein was significantly increased after treatment of tamsulosin, even as low as 50μM
concentration.
Conclusion: These results demonstrate that tamsulosin causes the cell death of NRP-152 cells, displaying
low concentration of tamsulosin induces apoptosis, but high concentration occurs necrosis. Bim, a proapoptotic
factor of the Bcl-2 family, expression was increased in the cells treated with tamsulosin, whereas Bcl-2 expression
was decreased. The present study suggests that clusterin may play a role in the process of apoptosis induced
by tamsulosin and Bim could be involved in the apoptosis. (J. Korean Continence Society 2006;10:132-139)
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참고문헌 (27)
참고문헌 신청
/ 1983 / In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastro-intestinal and respiratory tracts 53 : 155 ~ 61
/ 1983 / Ram rete testis fluid contains a protein(clusterin)which influences cell-cell interactions in vitro 28 : 1173 ~ 88
/ 1985 / Cellular response to androgen depletion and repletion in the rat ventral prostate:autoradiography and morphometric analysis 7 : 41 ~ 51
/ 1987 / Development and characteristics of the available animal model systems for the study of prostatic cancer 239 : 513 ~ 76
/ 1987 / R e s p o n s e o f g l a n d u l a r versus basal rat ventral prostatic epithelial cells to androgen withdrawal and replacement 11 : 229 ~ 42
/ 1983 / Ram rete testis fluid contains a protein(clusterin)which influences cell-cell interactions in vitro 28 : 1173 ~ 88
/ 1985 / Cellular response to androgen depletion and repletion in the rat ventral prostate:autoradiography and morphometric analysis 7 : 41 ~ 51
/ 1987 / Development and characteristics of the available animal model systems for the study of prostatic cancer 239 : 513 ~ 76
/ 1987 / R e s p o n s e o f g l a n d u l a r versus basal rat ventral prostatic epithelial cells to androgen withdrawal and replacement 11 : 229 ~ 42
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