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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
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In this study, Bacillus licheniformis which has been used as probiotics was isolated from Korean tradi-tional fermented soybean. A total of 69 strains were presumptively identified as B. licheniformis by phe-notypic methods. Based on PCR amplification and 16S rRNA gene sequencing, the multilocus sequence typing of gyrA and rpoB, followed by phylogenetic analysis was performed. The isolates were distinctly differentiated and found to be closely related to B. amyloliquefaciens, B. subtilis, and B. aerius. The partial 16S rRNA gene sequences of those strains matched those of B. sonorensis (97%) and B. aerius (98%) in the phylogenetic tree. In contrast, multilocus phylogenetic analysis (MLPA) showed that only 61 (86.9%) out of 69 strains were B. licheniformis. The rest of those strains were found to be B. subtilis (5.8%), B. amyloliquefaciens (2.9%), and B. sonorensis (2.9%), respectively. Therefore, our results sug-gested that since the 16S rRNA gene sequencing alone was not sufficient to compare and discriminate closely related lineages of Bacillus spp., it was required to analyze the MLPA simultaneously to avoid any misleading phenotype-based grouping of these closely related species.
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참고문헌 (22)
참고문헌 신청
Ash C / 1991 / Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences / Lett Appl Microbiol 13:202~206
Bernardeau M / 2013 / Overview of differences between microbial feed additives and probiotics for food regarding regulation, growth promotion effects and health properties and consequences for extrapolation of farm animal results to humans / Clin Microbiol Infect 19:321~330
Chun J / 2000 / Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences / Antonie Leeuwenhoek 78:123~127
Davis ME / 2008 / Effect of a Bacillusbased direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growing-finishing pigs / J Anim Sci 86:1459~1467
De Clerck E / 2004 / Genotypic diversity among Bacillus licheniformis strains from various sources / Fems Microbiology Letters 231:91~98
Bernardeau M / 2013 / Overview of differences between microbial feed additives and probiotics for food regarding regulation, growth promotion effects and health properties and consequences for extrapolation of farm animal results to humans / Clin Microbiol Infect 19:321~330
Chun J / 2000 / Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences / Antonie Leeuwenhoek 78:123~127
Davis ME / 2008 / Effect of a Bacillusbased direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growing-finishing pigs / J Anim Sci 86:1459~1467
De Clerck E / 2004 / Genotypic diversity among Bacillus licheniformis strains from various sources / Fems Microbiology Letters 231:91~98
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