지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 399 - 406 (8page)
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초록· 키워드
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Background: In this study, we evaluated the analytical performance and clinical potential of a one-step multiplex real-time PCR assay for the simultaneous detection of 14 types of respiratory viruses using the AdvanSure RV real-time PCR Kit (LG Life Sciences, Korea).
Methods: Three hundred and twenty clinical specimens were tested with the AdvanSure RV real-time PCR Kit and conventional multiplex reverse transcription (RT)-PCR assay. The assay results were analyzed and the one-step AdvanSure RV real-time PCR Kit was compared with the conventional multiplex RT-PCR assay with respect to the sensitivity and specificity of the detection of respiratory viruses.
Results: The limit of detection (LOD) was 1.31 plaque-forming units (PFU)/mL for human rhinoviruses (hRVs), 4.93 PFU/mL for human coronavirus HCoV-229E/NL63, 2.67 PFU/mL for human coronavirus HCoV-OC43, 18.20 PFU/mL for parainfluenza virus 1 (PIV)-1, 24.57 PFU/mL for PIV-2, 1.73 PFU/mL for PIV-3, 1.79 PFU/mL for influenza virus group (Flu) A, 59.51 PFU/mL for FluB, 5.46 PFU/mL for human respiratory syncytial virus (hRSV)-A, 17.23 PFU/mL for hRSV-B, 9.99 PFU/mL for human adenovirus (ADVs). The cross-reactivity test for this assay against 23 types of non-respiratory viruses showed negative results for all viruses tested. The agreement between the one-step AdvanSure multiplex real-time PCR assay and the conventional multiplex RT-PCR assay was 98%.
Conclusions: The one-step AdvanSure RV multiplex real-time PCR assay is a simple assay with high potential for specific, rapid and sensitive laboratory diagnosis of respiratory viruses compared to conventional multiplex RT-PCR.
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참고문헌 (24)
참고문헌 신청
Adcock PM / 1997 / Effect of rapid viral diagnosis on the management of children hospitalized with lower respiratory tract infection / Pediatr Infect Dis J 16 : 842 ~ 846
Bellau-Pujol S / 2005 / Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses / J Virol Methods 126 : 53 ~ 63
Boivin G / 2004 / Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses / J Clin Microbiol 42 : 45 ~ 51
Casiano-Colon AE / 2003 / Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults / J Clin Virol 28 : 169 ~ 174
Coiras MT / 2004 / Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays / J Med Virol 72 : 4
Bellau-Pujol S / 2005 / Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses / J Virol Methods 126 : 53 ~ 63
Boivin G / 2004 / Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses / J Clin Microbiol 42 : 45 ~ 51
Casiano-Colon AE / 2003 / Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults / J Clin Virol 28 : 169 ~ 174
Coiras MT / 2004 / Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays / J Med Virol 72 : 4
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