병아리 내이 유모세포에서 전압의존성 칼슘전류의 분리 측정

이승환; 신명철; 안태환; 김기용; 김국; 민현정; 박철원

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자료유형: 학술저널

저자정보: 이승환 신명철 안태환 김기용 김국 민현정 박철원

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제52권 제8호

발행연도: 2009.1

수록면: 655 - 659 (5page)

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Background and Objectives Voltage dependent calcium channel (VDCC) mediates calcium ion influx and controls neurotransmitter release in excitable cells. Hair cells in vertebrates cochlea are known to express L-type VDCC. The purpose of this study was to measure calcium current from hair cells to investigate basic activity and characteristics of VDCC. Materials and Method We measured calcium current in hair cells of the chicken’s auditory organ, the basilar papilla analogous to the mammalian cochlea, in whose L-type, dihydropyridinesensitive calcium channels predominate and in vestibular hair cells from cristae. Calcium current through VDCC was isolated in voltage-clamp recording using Cesium, Tetraethylammonium, 4- aminopyridine and apamin to block the much larger potassium currents. Various concentrations of internal calcium buffer, ethylene glycol tetraacetic acid (EGTA) or 1,2-bis (o-aminophenoxy) ethane-N,N,N’,N’-tetraacetic acid (BAPTA) were used. Results The higher the buffer concentration, the larger the current size were; they were significantly larger in 10 mM of calcium buffer concentration (ANOVA, p<0.05). There was no difference in calcium current between cochlear and vestibular hair cells. Conclusion We could successfully isolate stable inward calcium current from chick hair cells. This experiment can be used as a basic method to understand neurotransmission process between hair cells and afferent neurons.

#Hair cell·Calcium channel

참고문헌 (19)

참고문헌 신청

Brandt A / 2003 / CaV1.3 channels are essential for development and presynaptic activity of cochlear inner hair cells / J Neurosci 23 (34) : 10832 ~ 10840

Chen L / 1994 / Cochlear frequency-place map in adult chickens: intracellular biocytin labeling / Hear Res 81 (1) : 130 ~ 136

Fettiplace R / 1992 / The role of calcium in hair cell transduction / Soc Gen Physiol Ser (47) : 343 ~ 356

Fischer FP / 1992 / Quantitative analysis of the innervation of the chicken basilar papilla / Hear Res 61 (1) : 167 ~ 178

Fuchs PA / 1990 / Calcium currents in hair cells isolated from the cochlea of the chick / J Physiol (429) : 553 ~ 568

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이승환

소속기관 Department of Otolaryngology-Head and Neck Surgery, Hanyang University College of Medicine, Seoul, Korea