생쥐 경부림프절에서 면역 B 림프구의 특성

차창일; 김종빈; 김성완; 조중생; 여승근

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자료유형: 학술저널

저자정보: 차창일 김종빈 김성완 조중생 여승근

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제2호

발행연도: 2005.1

수록면: 241 - 246 (6page)

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Background and Objectives：To investigate the characteristic features of B cels in murine cervical lymph node. Animal ex-periment. Subjects and Method：We obtained cervical lymph node of mice. Isolated B cels were cultured in media after the cytometry. Imunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent asay. B20+ cells cultured for 5 days with media alone, or LPS. Entering into S phase in response to stimuli was measured by proliferative assay. Results：Identifying phenotypic characteristics for B cels of lymph node include CD5low, CD23high, CD43low, B20high, Ig (sIg)Mlow, sIgDhigh, CD80 ( )low, CD86 (B7.2)low, and Syndecam-1low. Spontaneous Imunoglobulin production did not occur in lymphoid B cels. When stimulation, IgM secretion was increased more than that of IgA and IgG. Lipopolysaccharide is a most powerful stimulus which secreted immunoglobulin. In thymidine incorporation, B cels were entered actively S phase after 48hours stimulation. CD40 plus anti-CD8 stimulated B cels to produce substantial thymidine incorporation that was much Conclusion：The B cels in cervical lymph node are conventional B cels (B2). Lymph node B cells secreted mainly IgM at stimuli and cell proliferation was activated at 48 hours of stimulation.

#B 림프구·생쥐·경부림프절. #B cel· Murine· Cervical lymph node.

참고문헌 (15)

참고문헌 신청

/ 1979 / Properties and application of monoclonal antibodies directedagainst determinants of the Thy-1 locus 122 : 2491 ~ 7

/ 1988 / Anti-Ig Ab inhibits the phorbol ester-inducedstimulation of peritoneal B cells 141 : 4089 ~ 93

/ 1988 / Regulation of cellmorphology in B lymphocytes by interleukin 4:Evidence for inducedcytoskeletal changes 160 : 5366 ~ 73

/ 1995 / Analysis of B-1 cell activation A Companion to Methods in Enzymology 1995 : 11 ~ 25

/ 1995 / Analysis of B-1 cell activation AComparison to Methods in Enzymology 1995 : 11 ~ 25

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