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Background and Objectives:This study was aimed to investigate the efects of 5-Hydroxytryptamine (5-HT) on the sponta-neous activity of medial vestibular nuclear neurons of the rat. Materials and Method:Sprague-Dawley rats aged 14 to 16 days neuron was obtained by micropunching. The disociated neurons were transferred into a recording chamber mounted on an inverted microscope and spontaneous action potentials were recorded by standard patch-clamp techniques. Results:The response of medial vestibular nuclear neurons to 5-HT applied in the recording chamber was not uniform. The spontaneous action potentials were increased in 10 cells, decreased in 3 cells and not altered in 2 of 15 cells. The spike width of the neurons was not afterhyperpolarization was decreased by 5-HT. Conclusion:These results suggest 5-HT increases spontaneous firing of the medial vestibular nuclear neurons by altering the afterhyperpolarization.

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