바퀴(Blattella germanica)에서 발현되는 세린계 단백분해효소 유전자군의 염기서열분석 및 알레르겐으로서의 가능성

이원용; 김현범; 박성현; 박경원; 옥미선; 유태현; 박종률

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자료유형: 학술저널

저자정보: 이원용 김현범 박성현 박경원 옥미선 유태현 박종률

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제51권 제6호

발행연도: 2008.1

수록면: 528 - 534 (7page)

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The serine protease allergens have been known as one of the most important inhalent allergens of insects as well as house dust mites. There is no known serine protease allergen of German cockroach, which is a well-known etiological agent of allergic diseases. We identified the serine protease genes of cockroach, Blattella germanica, and examined its possibility as an allergen. Materials and Method：We have isolated three partial genes, Bg3, Bg5 and Bg6, of serine protease from the Blattella germanica cDNA library using the degenerate oligonucleotide PCR primers specific for the conserved regions. Results：The genes, Bg3, Bg5 and Bg6, were composed of 396 bp 131 amino acids, 513 bp 170 amino acids, and 410 bp 136 amino acids, respectively. Northern hybridization analysis indicated that the size of Bg5 and Bg6 transcripts was approximately 0.7 kb. The gene, Bg5, was also identified as a multiple gene by the Southern blot analysis. The gene, Bg3, showed significant homology to trypsin-like serine protease of various insects. The deduced amino acids sequence of the gene Bg5 was matched to the mite group III allergen as well as various species of insect serine protease sequences. The gene Bg6 also showed high homology to the amino acids sequences of insect serine proteases and mite group III allergens. Three Blattella germanica serine protease gene fragments revealed close genetic relationships with Dermatophagoides farinae group III allergen in the phylogenetic analysis. Conclusion：The high homology and close phylogenetic relationship raise the possibility of three serine protease genes as being an allergen of German cockroach. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:528-34)

#Blattela germanica· Library of cDNA· PCR· Serine protease· Allergy.

참고문헌 (23)

참고문헌 신청

Bowles VM / 1988 / Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blowfly / Aust J Biol Res 41 (2) : 269 ~ 278

Casu RE / 1994 / Isolation of a trypsinlike serine protease gene family from the sheep blowfly Lucilia cuprina / Insect Mol Biol 3 (3) : 159 ~ 170

Davis CA / 1985 / A gene family in drosophila melanogaster coding for trypsin-like enzymes / Nucleic Acid Res 13 (18) : 6605 ~ 6619

Gaines PJ / 1999 / Cloning of a family of serine protease genes from the cat flea ctenocephalides felis / Insect Mol Biol 8 (1) : 11 ~ 22

Hong JH / 2004 / German cockroach extract activates protease-activated receptor 2 in human airway epithelial cells / J Allergy Clin Immunol 113 (2) : 315 ~ 319

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