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Background and Objectives:In head and neck cancer including hypopharyngeal carcinoma, cisplatin and 5-fluorouracil usualy have been used as neoadjuvant chemotherapeutic agents. We investigated the difference in the influences of cisplatin and 5-fluorouracil (5-FU) (cell cycle arest and apoptosis) in the hypopharyngeal cell line (PHUH-12). Method:PNUH-12 with a mutant type p53 (one point mutation at the 78th base, C to G, in exon 7) was treated with cisplatin and 5-FU. Changes in the cel line were assesed by MT assay, Western blotting (p53 and p21 protein), DNA fragmentation, PI stain, and DNA flow cytometry. Results:The p53 protein expresion was increased after the treatment in, we observed apoptosis by DNA fragmentation and PI stain and the increased S phase on DNA flow cytometry. But, with 5-FU, we couldnt observe apoptosis by DNA fragmentation, PI, and flow cytometry and only the increased G1 phase on DNA flow cytometry. Conclusion:In hypopharyngeal cell line (PNUH-12), cisplatin induced p53 dependent apoptosis and 5-FU induced p53 and p21 dependent G0/G1 cel cycle arest, but not apoptosis. (Korean J Otolaryngol 2002;45:984-9)

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