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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 241 - 247 (7page)
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초록· 키워드
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In an effort to discover alternative phytotherapeutic antimicrobial agents to combat Streptococcus parauberis, a fish pathogenic bacterium, we evaluated the antibacterial activity of seaweed extracts in vitro. A methanolic extract of Ecklonia cava exhibited strong antibacterial activity against S. parauberis isolated from olive flounder Paralichthys olivaceus. Furthermore, the n-hexane soluble (Hexane) fraction of the E. cava methanolic extract exhibited the greatest antibacterial effect on S. parauberis strains with a minimum inhibitory concentration (MIC) ranging from 256 to 1,024 μg/mL. In addition, the MIC values of oxytetracycline against antibiotic-resistant S. parauberis were markedly reduced up to 64-fold in combination with the Hexane fraction, suggesting that the antibacterial activity of the antibiotic was restored when combined with the Hexane fraction. The interaction between both antibiotics and the Hexane fraction was assessed by the fractional inhibitory concentration (FIC) index. The Hexane fraction and oxytetracycline combination against antibiotic-resistant S. parauberis strains resulted in a median ΣFIC range of 0.502 to 0.516. Thus, the synergistic ranges of median ΣFIC < 1 were observed for all combinations of the Hexane fraction and oxytetracycline against S. parauberis. To the best of our knowledge, this is the first report indicating the efficacy of an E. cava extract against fish pathogenic bacterium S. parauberis.
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참고문헌 (31)
참고문헌 신청
Adwan G / 2008 / Synergistic effect of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimen / Middle-East J Sci Res 3:134~139
백근욱 / 2006 / Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island / Journal of Veterinary Science 7(1):53~58
Bercovier H / 1997 / Fish Vaccinology. Dev Biol Stand / Karger :153~160
Choi JG / 2010 / Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp / Foodborne Path Dis 7:435~441
Clinical and Laboratory Standards Institute / Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Ninth Edition / CLSI document M07-A9
백근욱 / 2006 / Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island / Journal of Veterinary Science 7(1):53~58
Bercovier H / 1997 / Fish Vaccinology. Dev Biol Stand / Karger :153~160
Choi JG / 2010 / Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp / Foodborne Path Dis 7:435~441
Clinical and Laboratory Standards Institute / Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Ninth Edition / CLSI document M07-A9
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