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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1 - 8 (8page)
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The fractionation of serine protease inhibitor (SPI) from fish roe extracts was carried out using polyethylene glycol-4000 (PEG4000) precipitation. The protease inhibitory activity of extracts and PEG fractions from Alaska pollock (AP), bastard halibut (BH), skipjack tuna (ST), and yellowfin tuna (YT) roes were determined against target proteases. All of the roe extracts showed inhibitory activity toward bromelain (BR), chymotrypsin (CH), trypsin (TR), papain-EDTA (PED), and alcalase (AL) as target proteases. PEG fractions, which have positive inhibitory activity and high recovery (%), were the PEG1 fraction (0–5 %, w/v) against cysteine proteases (BR and PA) and the PEG4 fraction (20–40 %, w/v) against serine proteases (CH and TR). The strongest specific inhibitory activity toward CH and TR of PEG4 fractions was AP (9278 and 1170 U/mg) followed by ST (6687 and 2064 U/mg), YT (3951 and 1536 U/mg), and BH (538 and 98 U/mg). The inhibitory activity of serine protease in extracts and PEG fractions from fish roe was stronger than that of cysteine protease toward common casein substrate. Therefore, SPI is mainly distributed in fish roe and PEG fractionation effectively isolated the SPI from fish roes.
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참고문헌 (34)
참고문헌 신청
H. Akazawa / 1994 / Evaluation of Protease Inhibitors in Pacific Whiting Surimi / Journal of Aquatic Food Product Technology 2(3):79~95
Barret AJ. / 1986 / Proteinase inhibitors / Elsevier Science BV :3~22
T. Burnouf / 1995 / Chromatography in plasma fractionation: benefits and future trends / Journal of Chromatography B: Biomedical Sciences and Applications 664(1):3~15
Anas Cherqui / 2001 / Purification and characterization of two serine protease inhibitors from the hemolymph of Mythimna unipuncta / Insect Biochemistry and Molecular Biology 31(8):761~769
JUNG-HO CHOI / 2002 / Purification and characterization of a trypsin inhibitor from the egg of skipjack tuna Katsuwonus pelamis / Fisheries Science 68(6):1367~1373
Barret AJ. / 1986 / Proteinase inhibitors / Elsevier Science BV :3~22
T. Burnouf / 1995 / Chromatography in plasma fractionation: benefits and future trends / Journal of Chromatography B: Biomedical Sciences and Applications 664(1):3~15
Anas Cherqui / 2001 / Purification and characterization of two serine protease inhibitors from the hemolymph of Mythimna unipuncta / Insect Biochemistry and Molecular Biology 31(8):761~769
JUNG-HO CHOI / 2002 / Purification and characterization of a trypsin inhibitor from the egg of skipjack tuna Katsuwonus pelamis / Fisheries Science 68(6):1367~1373
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