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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 272 - 277 (6page)
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Seagrasses are marine angiosperms of ecological importance in providing shelter and food to aquatic species as well as maintaining the carbon cycle on earth. Phyllospadix iwatensis is a seagrass of the family Zosteraceae and is distributed along the eastern coast of Korea. The nucleotide sequences of P. iwatensis nuclear genes encoding 18S ribosomal RNA (rRNA) and internal transcribed spacer-1 (ITS-1) were determined for molecular phylogenetic analysis. Genomic DNA was isolated from P. iwatensis and used for PCR amplification of 18S rRNA and ITS-1. Examination of the 18S rRNA sequence of P. iwatensis showed a close (99% similarity) relationship to Zostera noltii, another genus of Zosteraceae, but a distant (84% similarity) evolutionary relationship to other macroalgal Laminariales species. Further discrepancies found in ITS-1 nucleotide sequences between closely related species indicate that the sequence information could be used for species identification.
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참고문헌 (20)
참고문헌 신청
Bird CJ / 1992 / The 18S rRNA gene sequences of four commercially important seaweeds / J Appl Phycol 4 : 379 ~ 384
Hoarau G / 2007 / A fast and inexpensive DNA extraction/purification protocol for brown macroalgae / Mol Ecol Notes 7 : 191 ~ 193
Koonjul PK / 1999 / Inclusion of polyvinylpyrrolidone in the poly-merase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA / Nucl Acids Res
Kuo J / 1990 / Fruit anatomy, seed germination and seedling development in the Japanese seagrass Phyllospadix (Zosteraceae) / Aquatic Bot 45 : 53 ~ 62
Nakajima M / 2000 / Rapid extraction of high-quality genomic DNA from Porphyra yezoensis (Bangiales, Rhodophyta) / Phycol Res 48 : 15 ~ 17
Hoarau G / 2007 / A fast and inexpensive DNA extraction/purification protocol for brown macroalgae / Mol Ecol Notes 7 : 191 ~ 193
Koonjul PK / 1999 / Inclusion of polyvinylpyrrolidone in the poly-merase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA / Nucl Acids Res
Kuo J / 1990 / Fruit anatomy, seed germination and seedling development in the Japanese seagrass Phyllospadix (Zosteraceae) / Aquatic Bot 45 : 53 ~ 62
Nakajima M / 2000 / Rapid extraction of high-quality genomic DNA from Porphyra yezoensis (Bangiales, Rhodophyta) / Phycol Res 48 : 15 ~ 17
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