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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 230 - 238 (9page)
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초록· 키워드
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Background : Mac-2 binding protein (Mac-2BP) is a secreted glycoprotein from the culture fluid of
several human cancer cells, especially breast, lung, and gastric cells. Mac-2BP plays a role in immune
response and cell adhesion activity in patients with various cancer and infectious diseases. In this
study, we attempted to identify the regulators of Mac-2BP expression at the transcriptional level.
Methods : To determine the effect of epidermal growth factor (EGF) to Mac-2BP expression in gastric
cancers, we constructed the different lengths of Mac-2BP promoter plasmids and measured the
promoter activity and Mac-2BP expression. In addition to investigating the role of signal transducer
and activator of transcription3 (STAT3) or human telomerase reverse transcriptase (hTERT) as a regulator
of Mac-2BP, we transfected the small interfering RNA (siRNA) specific for STAT3 or hTERT, and
Mac-2BP level was observed by a quantitative ELISA.
Results : EGF treatment could suppress the Mac-2BP transcription in HEK293 or gastric cancer cell
lines (SNU-638 or AGS). In 5′-deleted promoter experiment, pGL3-Mac Pro-2377 transfected cells
showed a decreased luciferase activity compared to pGL3-Mac Pro-2277. We also identified that
(-2,366/-2,356) on Mac-2BP promoter is a putative STAT3 binding site and suppression of STAT3
with STAT3 specific siRNA increased the Mac-2BP level, suggesting the role of STAT3 as a negative
regulator, in contrast to hTERT, which is known as a positive regulator.
Conclusions : EGF signal is critical for the Mac-2BP expression, and more importantly, STAT3 could
work as a negative regulator, while hTERT as a positive regulator in Mac-2BP transcription. (Korean
J Lab Med 2008;28:230-8)
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Akira S / 1994 / Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related transcription factor involved in the gp130-mediated signaling pathway / Cell 77
Bowman T / 2000 / STATs in oncogenesis / Oncogene 19 : 2474 ~ 2488
Bowman T / 2001 / Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis / Proc Natl Acad Sci USA 98 : 7319 ~ 7324
Bromberg J / 2002 / Stat proteins and oncogenesis / J Clin Invest 109 : 1139 ~ 1142
Bromberg JF / 1999 / Albanese C, et al. Stat3 as an oncogene / Cell 98 : 295 ~ 303
Bowman T / 2000 / STATs in oncogenesis / Oncogene 19 : 2474 ~ 2488
Bowman T / 2001 / Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis / Proc Natl Acad Sci USA 98 : 7319 ~ 7324
Bromberg J / 2002 / Stat proteins and oncogenesis / J Clin Invest 109 : 1139 ~ 1142
Bromberg JF / 1999 / Albanese C, et al. Stat3 as an oncogene / Cell 98 : 295 ~ 303
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