지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 1,009 - 1,022 (14page)
이용수
초록· 키워드
오류제보하기
Purpose Glioblastoma (GBM) is classified as one of the most aggressive and lethal brain tumor. Great strides have been made in understanding the genomic and molecular underpinnings of GBM, which translated into development of new therapeutic approaches to combat such deadly disease. However, there are only few therapeutic agents that can effectively inhibit GBM invasion in a clinical framework. In an effort to address such challenges, we have generated anti-SEMA3A monoclonal antibody as a potential therapeutic antibody against GBM progression. Materials and Methods We employed public glioma datasets, Repository of Molecular Brain Neoplasia Data and The Cancer Genome Atlas, to analyze SEMA3AmRNA expression in human GBM specimens. We also evaluated for protein expression level of SEMA3A via tissue microarray (TMA) analysis. Cell migration and proliferation kinetics were assessed in various GBM patient-derived cells (PDCs) and U87-MG cell-line for SEMA3A antibody efficacy. GBM patient-derived xenograft (PDX) models were generated to evaluate tumor inhibitory effect of anti-SEMA3A antibody in vivo. Results By combining bioinformatics and TMA analysis, we discovered that SEMA3A is highly expressed in human GBM specimens compared to non-neoplastic tissues. We developed three different anti-SEMA3A antibodies, in fully human IgG form, through screening phagedisplayed synthetic antibody library using a classical panning method. Neutralization of SEMA3A significantly reduced migration and proliferation capabilities of PDCs and U87-MG cell line in vitro. In PDX models, treatment with anti-SEMA3A antibody exhibited notable tumor inhibitory effect through down-regulation of cellular proliferative kinetics and tumorassociated macrophages recruitment. Conclusion In present study, we demonstrated tumor inhibitory effect of SEMA3A antibody in GBM progression and present its potential relevance as a therapeutic agent in a clinical framework.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Adamson C / 2009 / Glioblastoma multiforme: a review of where we have been and where we are going / Expert Opin Investig Drugs 18:1061~1083
Louis DN / 2007 / The 2007 WHO classification of tumours of the central nervous system / Acta Neuropathol 114:97~109
Reardon DA / 2006 / Therapeutic advances in the treatment of glioblastoma: rationale and potential role of targeted agents / Oncologist 11:152~164
Stupp R / 2005 / Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma / N Engl J Med 352:987~996
Linger RM / 2008 / TAM receptor tyrosine kinases: biologic functions, signaling, and potential therapeutic targeting in human cancer / Adv Cancer Res 100:35~83
Louis DN / 2007 / The 2007 WHO classification of tumours of the central nervous system / Acta Neuropathol 114:97~109
Reardon DA / 2006 / Therapeutic advances in the treatment of glioblastoma: rationale and potential role of targeted agents / Oncologist 11:152~164
Stupp R / 2005 / Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma / N Engl J Med 352:987~996
Linger RM / 2008 / TAM receptor tyrosine kinases: biologic functions, signaling, and potential therapeutic targeting in human cancer / Adv Cancer Res 100:35~83
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Concurrent Anti-glomerular Basement Membrane Nephritis and IgA Nephropathy
Journal of Pathology and Translational Medicine
2019 .01
-
In vivo modeling of glioma using genome editing of neural stem cells: From clinical insight to translational research
한국실험동물학회 학술발표대회 논문집
2021 .07
-
교모세포종에 대한 통합암치료의 치료 효과 증례보고
대한한방내과학회지
2022 .05
-
Roles of Valproic Acid in Improving Radiation Therapy for Glioblastoma: a Review of Literature Focusing on Clinical Evidence
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Polymorphism in the Promoter Region of SEMA5A Is Associated with Sociality Traits in Korean Subjects with Autism Spectrum Disorders
PSYCHIATRY INVESTIGATION
2017 .01
-
Mesenchymal Stem-Like Cells Derived from the Ventricle More Effectively Enhance Invasiveness of Glioblastoma Than Those Derived from the Tumor
Yonsei Medical Journal
2023 .03
-
Astrocytic scar restricting glioblastoma via glutamate–MAO-B activity in glioblastoma-microglia assembloid
생체재료학회지
2023 .03
-
Multifunctional lipidic nanocarriers for effective therapy of glioblastoma: recent advances in stimuli-responsive, receptor and subcellular targeted approaches
Journal of Pharmaceutical Investigation
2022 .01
-
Sema4D deficiency improved metabolic phenotype through immature B cell development in diet-induced obeesity
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Anti-H Antibody Showing Agglutination in the Anti-Human Globulin Phase in a Korean Patient
Annals of Laboratory Medicine
2016 .01
-
Evaluation of plant-derived antibody potential for HER2-targeted imaging and therapeutic efficacy using breast cancer animal models
한국실험동물학회 학술발표대회 논문집
2023 .02
-
Immunohistochemical Analysis of ATRX, IDH1 and p53 in Glioblastoma and Their Correlations with Patient Survival
Journal of Korean Medical Science
2016 .01
-
Unusual Case of Posterior Reversible Encephalopathy Syndrome in a Patient with Anti-glomerular Basement Membrane Antibody Glomerulonephritis: A Case Report and Review of the Literature
Electrolytes & Blood Pressure
2017 .01
-
Current Status of External Quality Control for the Classification of Complex Antibodies on the Unexpected Antibody Identification Test
Journal of Laboratory Medicine And Quality Assurance
2021 .12
-
Effect of glucose level on chemical hypoxia- and hydrogen peroxide-induced chemokine expression in human glioblastoma cell lines
The Korean Journal of Physiology & Pharmacology
2017 .09
-
Application of Single-Domain Antibodies (“Nanobodies”) to Laboratory Diagnosis
Annals of Laboratory Medicine
2021 .11
-
Application of Volumetric Analysis to Glioblastomas: a Correlation Study on the Status of the Isocitrate Dehydrogenase Mutation
Investigative Magnetic Resonance Imaging
2015 .01
-
Underexpression of HOXA11 Is Associated with Treatment Resistance and Poor Prognosis in Glioblastoma
Cancer Research and Treatment
2017 .01
-
In vivo imaging of Tumor-associated Macrophages to Delineate the Margins of Glioblastoma using Near-infrared Fluorescent Nanoparticles
한국실험동물학회 학술발표대회 논문집
2016 .08
-
Bortezomib-Containing Multimodality Treatment for Antibody-Mediated Rejection with Anti-HLA and Anti-AT1R Antibodies after Kidney Transplantation
Yonsei Medical Journal
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0